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Protein isolation

How can you isolate any protein with Western blot? :roll: Trizol should work for protein extraction, but I have never used it. I guess they will be inactive if is it OK for you.

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by JackBean
Wed Feb 13, 2013 1:13 pm
Forum: Molecular Biology
Topic: Protein isolation
Replies: 2
Views: 2613

Animal cell nucleus shape changes

... So perhaps a better term would be "dynamic, but defined", which is almost the same as "fixed" if the cell is mature and/or inactive. Mitosis is a little peculiar case, since during it the nuclear membrane briefly disappears and is re-assmbled for both of the daughter cells ...

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by biohazard
Mon Nov 12, 2012 2:04 pm
Forum: Cell Biology
Topic: Animal cell nucleus shape changes
Replies: 7
Views: 12188

How dominance works at the DNA/RNA/protein

... However, the g trait might not get translated, in which case we will only see proteins that lead to the G trait. Also, the g proteins may be inactive. These are two more mechanisms that could cause G to be dominant and g recessive. Going further back in time, the sperm that carried the g ...

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by mhwombat
Thu Oct 04, 2012 6:35 pm
Forum: Genetics
Topic: How dominance works at the DNA/RNA/protein
Replies: 10
Views: 9795

EC50 depending on cell number?

... of compound in the well. Again, to me this feels wrong but I cannot really explain why - Say a compound has an e.g. EC50 of 1 mM (so a quite inactive compound) in a standard assay with 10.000 cells per well with 100ul medium volume. If theoretically I now have 1 cell per well in 1ml volume, ...

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by qforthemass
Tue Aug 14, 2012 9:40 pm
Forum: Cell Biology
Topic: EC50 depending on cell number?
Replies: 1
Views: 3469

Re: Can you explain the Ras recruitment system?

... is already fused to the plasma membrane. My questions are: 1) is the cdc25-2 yRAS mutant already attached to the plasma membrane, even in the inactive GDP-associated form? 2) How can we tell what the switch is? Can't the bait interact with unindented proteins on the yeast cell membrane? Isn't ...

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by janapix
Mon Jun 11, 2012 3:33 am
Forum: Molecular Biology
Topic: Can you explain the Ras recruitment system?
Replies: 1
Views: 2913
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