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Inactivated serum


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Blood Clotting

Blood does not clot if there are no clotting factors, or they are inactivated. A drop of blood on the floor will dry, not clot. If the blood ... factors the blood cells will simply sediment and clear plasma or serum will remain on top of the cells. Erythrocytes, being the heaviest, will ...

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by biohazard
Mon Aug 30, 2010 6:08 am
 
Forum: Human Biology
Topic: Blood Clotting
Replies: 3
Views: 4048

Re: FBS store

First of all, like other have said, I think the serum should be fine left out overnight. However, I'll state that I have cultured cells for a long time without having "inactivated" the FBS serum. Additionally, I believe the term inactivation is a misnomer ...

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by MJD
Thu Nov 05, 2009 9:12 am
 
Forum: Cell Biology
Topic: FBS store
Replies: 11
Views: 10072

Re: Cell Death after selection pressure has been removed?

... it is no mystery after all. I have screened the several batches of heat inactivated serum i have been using, and one of them turned out to be just way bad. The same one I used ...

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by Ilsalmi
Wed May 21, 2008 8:54 pm
 
Forum: Cell Biology
Topic: Cell Death after selection pressure has been removed?
Replies: 2
Views: 2433

Dispersing Agents For Cell Cultures

... After the cells have lost their adhesion to the flask, the trypsin is inactivated by adding some culture medium with fetal serum. Now, if you don't inactivate the trypsin the cells will not all die, however some of ...

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by MrMistery
Wed Mar 05, 2008 5:13 pm
 
Forum: Cell Biology
Topic: Dispersing Agents For Cell Cultures
Replies: 2
Views: 2802

The Fiber Disease

... Samples were incubated at 55°C overnight, and the proteinase k was inactivated at 95°C for 10 minutes and then subjected to two freeze-thaw ... chamber. The hybridization buffer consisted of 10% dextran, 0.2% bovine serum albumin, and 0.01% polyadenosine, in 5X SET buffer; the 25X SET buffer ...

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by al
Mon Dec 25, 2006 1:39 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5409507
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