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Inactivated serum


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Re: Cell Death after selection pressure has been removed?

... it is no mystery after all. I have screened the several batches of heat inactivated serum i have been using, and one of them turned out to be just way bad. The same one I used ...

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by Ilsalmi
Wed May 21, 2008 8:54 pm
 
Forum: Cell Biology
Topic: Cell Death after selection pressure has been removed?
Replies: 2
Views: 903

Dispersing Agents For Cell Cultures

... After the cells have lost their adhesion to the flask, the trypsin is inactivated by adding some culture medium with fetal serum. Now, if you don't inactivate the trypsin the cells will not all die, however some of ...

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by MrMistery
Wed Mar 05, 2008 5:13 pm
 
Forum: Cell Biology
Topic: Dispersing Agents For Cell Cultures
Replies: 2
Views: 592

The Fiber Disease

... Samples were incubated at 55°C overnight, and the proteinase k was inactivated at 95°C for 10 minutes and then subjected to two freeze-thaw ... chamber. The hybridization buffer consisted of 10% dextran, 0.2% bovine serum albumin, and 0.01% polyadenosine, in 5X SET buffer; the 25X SET buffer ...

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by al
Mon Dec 25, 2006 1:39 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 748735

The Fiber Disease

... When this was checked out, the state denied ever receiving any serum, let alone "monitoring" any for him. One patient filed a ... trying to reach him since have reported that his voicemail has been inactivated. There is a "Stealth Virus" group on the internet where ...

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by Linn
Sun Apr 16, 2006 9:55 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 748735

medium for mammalian cells

Might you mean complete and serum FREE media? Complete media is a complex, undefined mix of things usually containing ... a mixture of proteins / hormones / growth factors etc (you can also get heat inactivated serum in which all the proteins have been denatured). The problem with this ...

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by DevGrp
Wed Mar 16, 2005 11:02 am
 
Forum: Cell Biology
Topic: medium for mammalian cells
Replies: 8
Views: 2918


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