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Inactivate

Inactivate

to destroy the biological activity or the effects of an agent or substance, as the activity of complement is destroyed when serum is heated.


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Re: Any ideas for vaccine development or anti-viral drugs???

... Chemother. 2009 Feb 17. [Epub ahead of print]. Phosphorodiamidate morpholino oligomers (PMO) are uncharged nucleic acid-like molecules designed to inactivate specific gene expression via antisense-based steric hindrance of mRNA translation. PMOs have been successful in knockdown of viral gene expression ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 504

Females heterozygous for X linked diseases

I know that female mammalians randomly inactivate one X chromosome in somatic cells.So, if a human female was daltonism carrier, would not she be half daltonic?Because, ~1/2 of her cells in retina would have a recessive allele and the other ...

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by kysa
Fri Jan 04, 2013 5:04 pm
 
Forum: Genetics
Topic: Females heterozygous for X linked diseases
Replies: 1
Views: 1394

Re: Inactivate an enzyme

You must inactivate it before ligation. After ligation is the phosphorylase harmless. :roll: Expect low yield, if you'll get something at all.

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by JackBean
Thu Apr 12, 2012 8:39 am
 
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 2037

Re: Inactivate an enzyme

The enzyme could do something if the DNA insert is not dephosphorylased, so what shall i do ? cause i don't need to dephosphorylate the insert.

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by foufoulette
Wed Apr 11, 2012 12:35 pm
 
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 2037

Inactivate an enzyme

Hi everyone ! Please, i need your opignon about what i've done. Is it correct to don't inactivate enzyme (phosphatase) after a diphosphatasing of a plasmid and do the next step which is ligation, without inactivation ? is this could affect my results ?

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by foufoulette
Wed Apr 11, 2012 1:53 am
 
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 2037
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