Inactivate

I know that female mammalians randomly inactivate one X chromosome in somatic cells.So, if a human female was daltonism carrier, would not she be half daltonic?Because, ~1/2 of her cells in retina would have a recessive allele and the other ...

See entire post

You must inactivate it before ligation. After ligation is the phosphorylase harmless. :roll: Expect low yield, if you'll get something at all.

See entire post

The enzyme could do something if the DNA insert is not dephosphorylased, so what shall i do ? cause i don't need to dephosphorylate the insert.

See entire post

Hi everyone ! Please, i need your opignon about what i've done. Is it correct to don't inactivate enzyme (phosphatase) after a diphosphatasing of a plasmid and do the next step which is ligation, without inactivation ? is this could affect my results ?

See entire post

Hello!! and how can enzyme inhibitors irreversibly inactivate enzymes??

See entire post