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Inactivate

Inactivate

to destroy the biological activity or the effects of an agent or substance, as the activity of complement is destroyed when serum is heated.


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Females heterozygous for X linked diseases

I know that female mammalians randomly inactivate one X chromosome in somatic cells.So, if a human female was daltonism carrier, would not she be half daltonic?Because, ~1/2 of her cells in retina would have a recessive allele and the other ...

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by kysa
Fri Jan 04, 2013 5:04 pm
 
Forum: Genetics
Topic: Females heterozygous for X linked diseases
Replies: 1
Views: 1319

Re: Inactivate an enzyme

You must inactivate it before ligation. After ligation is the phosphorylase harmless. :roll: Expect low yield, if you'll get something at all.

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by JackBean
Thu Apr 12, 2012 8:39 am
 
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 1907

Re: Inactivate an enzyme

The enzyme could do something if the DNA insert is not dephosphorylased, so what shall i do ? cause i don't need to dephosphorylate the insert.

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by foufoulette
Wed Apr 11, 2012 12:35 pm
 
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 1907

Inactivate an enzyme

Hi everyone ! Please, i need your opignon about what i've done. Is it correct to don't inactivate enzyme (phosphatase) after a diphosphatasing of a plasmid and do the next step which is ligation, without inactivation ? is this could affect my results ?

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by foufoulette
Wed Apr 11, 2012 1:53 am
 
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 1907

Re: enzyme activity, please help

Hello!! and how can enzyme inhibitors irreversibly inactivate enzymes??

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by Trevorwin
Fri Sep 16, 2011 7:28 am
 
Forum: Cell Biology
Topic: enzyme activity, please help
Replies: 6
Views: 7996
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