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In vitro

In vitro

within a glass, observable in a test tube, in an artificial environment. In an artificial environment outside the living organism; an egg fertilized in vitro.In an artificial environment outside the living organism; in vitro fertilization.Biological processes or reactions that would normally occur within an organism but are made to occur in an artificial environment, i.e. A laboratory. See in vivo.

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Human Gamete Compatibility Going Backwards

Depends how you want to go about doing it. From in vitro stand point the main requirement is same number of chromosomes - therefore, Reeves's Muntjac and Sable Antelope could be fully "compatible" with humans. I don't think anyone tested ...

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by Cat
Sun Jan 05, 2014 5:40 pm
Forum: Evolution
Topic: Human Gamete Compatibility Going Backwards
Replies: 9
Views: 4388

How long will myocytes survive in plasma+saline?

I'm going to a farm this weekend to watch how they slaughter pigs. I've kept myocytes in-vitro with DMEM or the likes a few years ago in college, as trypsinized adhesion culture and explant chunk from chicken embryo. I've also made hybridomas from mice, so I know about ...

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by nmz787
Tue Oct 15, 2013 9:26 pm
Forum: Cell Biology
Topic: How long will myocytes survive in plasma+saline?
Replies: 0
Views: 884

Re: Sound and how the brain reacts to it

... they excite vibrations below the thermal noise background in the ear. The answer may be active amplification. Previous experiments performed in vitro found that bundles oscillate spontaneously from an internal driver, and this motion could synchronize with a small external input. Yuttana Roongthumskul ...

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by GremlinGnome422
Mon Jul 01, 2013 7:25 pm
Forum: Human Biology
Topic: Sound and how the brain reacts to it
Replies: 89
Views: 142287

Cell Signaling

When one studies the protein protein interaction, why it is sometimes recommended to perform in vitro linked transcription translation, when immunoprecipitation anyhow confirms your in vivo results. I have done this in my masters thesis but have been asked by many that ...

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by Shaanu
Mon Jun 17, 2013 9:04 am
Forum: Cell Biology
Topic: Cell Signaling
Replies: 1
Views: 900

Re: In Vitro Transcription Translation

It sounds like the autoradiography is done directly on the gel. Since the protein contains the hot sulfur, you don't need a radiolabeled antibody to detect it. You can infer the mass of the protein from its mobility (location of gel band). However, if you need to unambiguously prove the identity of ...

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by jonmoulton
Wed Jun 05, 2013 10:59 pm
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 1973
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