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In situ hybridization

Definition

noun

A type of hybridization that detects and localizes the presence of specific DNA sequence within tissue samples (‘’in situ’’) when a labeled DNA or RNA probe binds with it by complementary base pairing.


Supplement

DNA ISH can be used to determine the structure of chromosomes. Fluorescent DNA ISH (FISH) can, for example, be used in medical diagnostics to assess chromosomal integrity. RNA ISH (hybridization histochemistry) is used to measure and localize mRNAs and other transcripts within tissue sections or whole mounts.


‘’Abbreviation:’’ ISH

‘’Alternative form:’’ ‘’in situ’’ hybridisation

‘’Related terms:’’


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microRNA in situ hybridization protocol: zebrafish

... AK, Moulton JD, Bakkers J. Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues. Biology Open. 2012;[Advance ...

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by jonmoulton
Fri Apr 27, 2012 4:32 pm
 
Forum: Molecular Biology
Topic: microRNA in situ hybridization protocol: zebrafish
Replies: 0
Views: 1049

In situ RNA-RNA probes

hi, i am new to use in situ hybridization i made in situ RNA probes from PCR template and T7 RNA polymerase, the produced probes has 1.5ug/ul ...

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by yungyungbee
Thu Mar 04, 2010 8:33 am
 
Forum: Molecular Biology
Topic: In situ RNA-RNA probes
Replies: 0
Views: 978

Anybody knows how stable is ssDNA??

Hi guys, I'm preparing the probe for in situ hybridization. It's the product from degenerated PCR so i guess it's ssDNA. I labeled the probe with ...

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by primary
Tue Jun 09, 2009 11:08 pm
 
Forum: Molecular Biology
Topic: Anybody knows how stable is ssDNA??
Replies: 2
Views: 1763

Lithium Chloride solution for RNA precipitation

... too embarassed to ask my labmates. The lab protocol for producing in situ hybridization probes calls for precipitation in lithium chloride solution in order to "clean ...

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by snowcapk
Wed Mar 26, 2008 7:44 am
 
Forum: Molecular Biology
Topic: Lithium Chloride solution for RNA precipitation
Replies: 0
Views: 3927

One question about Fluorescence in situ Hybridization(FISH)

All protocal suggest that Fluorescence in situ Hybridization(FISH) need humannity chamber. Is this instrument necessary? and why? I want to use BAC ...

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by zeyuanzhu
Mon Nov 05, 2007 1:10 am
 
Forum: Molecular Biology
Topic: One question about Fluorescence in situ Hybridization(FISH)
Replies: 0
Views: 1288
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