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In situ hybridization

Definition

noun

A type of hybridization that detects and localizes the presence of specific DNA sequence within tissue samples (‘’in situ’’) when a labeled DNA or RNA probe binds with it by complementary base pairing.


Supplement

DNA ISH can be used to determine the structure of chromosomes. Fluorescent DNA ISH (FISH) can, for example, be used in medical diagnostics to assess chromosomal integrity. RNA ISH (hybridization histochemistry) is used to measure and localize mRNAs and other transcripts within tissue sections or whole mounts.


‘’Abbreviation:’’ ISH

‘’Alternative form:’’ ‘’in situ’’ hybridisation

‘’Related terms:’’


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Comprehensive List of long noncoding RNA(lncRNA) databases

... long ncRNAs in human and mouse. It also contains both microarray and in situ hybridization data, much of which is described here for the first time. website: http://jsm-research.imb.uq.edu.au/nred/

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by rnaworld2110
Fri Dec 14, 2012 6:07 am
 
Forum: Molecular Biology
Topic: Comprehensive List of long noncoding RNA(lncRNA) databases
Replies: 2
Views: 2458

microRNA in situ hybridization protocol: zebrafish

... AK, Moulton JD, Bakkers J. Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues. Biology Open. 2012;[Advance ...

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by jonmoulton
Fri Apr 27, 2012 4:32 pm
 
Forum: Molecular Biology
Topic: microRNA in situ hybridization protocol: zebrafish
Replies: 0
Views: 992

In situ RNA-RNA probes

hi, i am new to use in situ hybridization i made in situ RNA probes from PCR template and T7 RNA polymerase, the produced probes has 1.5ug/ul ...

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by yungyungbee
Thu Mar 04, 2010 8:33 am
 
Forum: Molecular Biology
Topic: In situ RNA-RNA probes
Replies: 0
Views: 962

Anybody knows how stable is ssDNA??

Hi guys, I'm preparing the probe for in situ hybridization. It's the product from degenerated PCR so i guess it's ssDNA. I labeled the probe with ...

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by primary
Tue Jun 09, 2009 11:08 pm
 
Forum: Molecular Biology
Topic: Anybody knows how stable is ssDNA??
Replies: 2
Views: 1729

Lithium Chloride solution for RNA precipitation

... too embarassed to ask my labmates. The lab protocol for producing in situ hybridization probes calls for precipitation in lithium chloride solution in order to "clean ...

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by snowcapk
Wed Mar 26, 2008 7:44 am
 
Forum: Molecular Biology
Topic: Lithium Chloride solution for RNA precipitation
Replies: 0
Views: 3896
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