Dictionary » I » In situ hybridisation

In situ hybridisation



A type of hybridization that detects and localizes the presence of specific DNA sequence within tissue samples (‘’in situ’’) when a labeled DNA or RNA probe binds with it via complementary base pairing.


DNA ISH can be used to determine the structure of chromosomes. Fluorescent DNA ISH (FISH) can, for example, be used in medical diagnostics to assess chromosomal integrity. RNA ISH (hybridization histochemistry) is used to measure and localize mRNAs and other transcripts within tissue sections or whole mounts.

‘’Abbreviation:’’ ISH

‘’Alternative form:’’ ‘’in situ’’ hybridization

‘’Related terms:’’

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Fc fusion probes

... Fc/AP fusion probes work? Can you use them as you would in a normal in situ hybridisation to see expression patterns? Also what are the benefits to using them? I've tried ...

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by sophie1983
Thu Nov 19, 2009 9:42 am
Forum: Molecular Biology
Topic: Fc fusion probes
Replies: 3
Views: 1199

clearing up terminology

... on the molecular anaylsis advancements of histopathology such as in situ hybridisation and in situ PCR. however when i look these methods up, they fall under cytogenetics ...

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by aftereleven
Fri Jan 04, 2008 5:15 pm
Forum: Molecular Biology
Topic: clearing up terminology
Replies: 1
Views: 1583


... some one please explain in laymans terms what the principle of RNA in situ hybridisation is please? I'm getting confused! Thanks

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by Charlie
Sun Dec 04, 2005 3:04 pm
Forum: Cell Biology
Topic: Hybridisation
Replies: 0
Views: 1000

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