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Immunohistochemistry

Immunohistochemistry

Histochemical localization of immunoreactive substances using labelled antibodies as reagents.


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Relationship between cell lines and cancer research (IHC)

... question in the wrong section. At my current internship I am going to stain different cell lines (examples: HELAp2, COLO741, etc) with antigens (immunohistochemistry) relating to cancer research (examples p53, MSH-2/6, MLH-1, PMS-2). But I can’t seem to understand the underlying reason why we ...

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by wptmdoorn
Tue Aug 27, 2013 11:39 am
 
Forum: Cell Biology
Topic: Relationship between cell lines and cancer research (IHC)
Replies: 2
Views: 531

Re: Difference between ICC and IF

This is how immunohistochemistry is typically done, so that is probably enough of a name. However, you could specify the antibodies, for example: immmunihistochemistry using goat anti-VEGFR primary antibody and anti-goat-IgG secondary ...

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by jonmoulton
Mon Feb 25, 2013 5:11 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 14381

Re: Cytokine - Receptor concepts

... biological experiments to determine the localization and distribution of your interested receptors with purchased specifc antibodies. For example, immunohistochemistry is a useful rechnique. To get a reference, you can visit http://www.immunohistochemistry.us/

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by sunhan1985
Fri Dec 21, 2012 2:27 am
 
Forum: Cell Biology
Topic: Cytokine - Receptor concepts
Replies: 7
Views: 13174

Queston about Zebrafish Whole mount Immunohistochemistry?

Hello, Has anyone done an IHC experiment in Zebrafish embryos for detecting 8-oxoG? I was wondering what would be the best fixative to use? Thanks

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by Vivkrish
Tue Apr 24, 2012 7:58 pm
 
Forum: Molecular Biology
Topic: Queston about Zebrafish Whole mount Immunohistochemistry?
Replies: 0
Views: 811

Re: Cytokine - Receptor concepts

... are being degraded by proteases during normal protein turnover and finally only one is left. Receptor concentrations can be roughly estimated by immunohistochemistry (e.g. fluorescent antibodies) but are more rigorously quantitated by Western blot (which requires a larger sample, more cells). ...

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by jonmoulton
Wed Jun 02, 2010 5:26 pm
 
Forum: Cell Biology
Topic: Cytokine - Receptor concepts
Replies: 7
Views: 13174
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