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I band

I band

(Science: cell biology) The isotropic band of the sarcomere of striated muscle, where only thin filaments are found. Unlike the a Band, the i band can vary in width depending upon the state of contraction of the muscle when fixed.


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separation problem with GelRed

... Therefore, its large size may affect DNA migration in prestain gels, leading to some separation problems. Here are some solutions to improve band separation: 1. Reduce the amount of loaded DNA. Overloading of DNA often results in band smearing. The recommended amount for ladders and samples ...

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by Biotium
Wed Nov 26, 2014 4:43 pm
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 19556

pfge problem H.influenzae

... doing PFGE with Haemophilus influenzae. Also i am using SmaI restriction enzyme, but i get this profile. Not as smear, actually i am counting many bands but as we know we have to find minumum 8 bands. Any idea about band profile ? Is this a good profile? Thank you

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by biologistesab
Sun Nov 09, 2014 11:14 am
 
Forum: Molecular Biology
Topic: pfge problem H.influenzae
Replies: 0
Views: 466

Souther blot and restriction mapping

... to draw several lines each with marking the placeswhere the enzymes must cut and space in between X must be Xho obviously as it gives exactly one band of the same size so X + E gives |-------0,7----|-----------1,7----------| X + B gives |------2,3--------------------|---0,1--| B + E gives |-----------2,0---------|------------1,6-------|----0,2------| ...

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by JackBean
Wed Oct 08, 2014 9:57 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 1
Views: 1584

Souther blot and restriction mapping

... The probe was a 2.4 kb XhoI fragment. Using the southern data, determine the restriction map for genomic DNA. Data: Enzyme Approximate Band Sizes E ------------- 6.0 ----- 1.8 X ------------- 2.4 B ------------ >10 ----- 3.6 XE ------------ 1.7 ----- 0.7 XB ------------ 2.3 ----- ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 1
Views: 1584

Re: An interesting PCR smearing...I dont know what to do :(

... a similar problem. I am using 3 different primers to amplify 3 different exons of a gene . 1 of the exon is amplifying very well ( a single bright band ) but the other two exon amplification is not coming properly.and this has occured recently. ( even though the PCR reaction was standardized earlier ...

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by priyah
Sat Jun 28, 2014 5:20 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 8772
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