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How different about DNA amplified band and polymorphic band? I would like to calcualte %of polymorphic band after PCR and electrophoresis, please explian. Thank you very much

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... then for 4 months or more I look like I am 6 months pregnant. I can not wear pant that I wore 2 months ago. I have to wear a maternity belly band over my pant to hold them up the put a big shirt over it so I can have something to wear to work. I have been tested fro thyroid, gluten and everything ...

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Hi! Could you describe the experiment more detailed? where do the primers anneal? why are there two bands? did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the mutated site? I don't think that contamination is the ...

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how big were the differnces in size? If they are very big maybe you just ran the gel too fast or too long and the smaller band ran off the gel.

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... trying to determine the genotype of some cell samples using PCR and agaorse gel electrophoresis. On one of the samples, I was supposed to find two bands representing a heterozygote for a particular mutated receptor but I could only see one band representing the wild type homozygote. When other ...

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