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Hydroxide ion

Hydroxide ion

the anion OH having one oxygen and one hydrogen atom, i.e. OH-


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Re: hydroxyproline assay to determine collagen content

... - 1-5 µg/2 0.001N HCl ml. Buffer-0.5 g citric acid monohydrate, 0.12 ml glacial acetic acid, 0.82 g sodium acetate anhydrous, and 0.34 g sodium hydroxide are combined and the final volume is brought to 10 ml with distilled water. Make sure the pH is 6.0. Store in the refrigerator with aluminum ...

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by ucsfmed2012
Thu Oct 27, 2011 9:30 am
 
Forum: Molecular Biology
Topic: hydroxyproline assay to determine collagen content
Replies: 5
Views: 8251

Deproteinizing plasma?

Does anyone have a step by step protocol for deproteinizing plasma for glucose with barium hydroxide and sulfate?

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by ntb103
Mon Jan 18, 2010 5:48 pm
 
Forum: Physiology
Topic: Deproteinizing plasma?
Replies: 10
Views: 9461

Help with my Beetroot Lab

The point of my links was, that these colors and their colors are pH-dependent, so if you use acid or hydroxide, the color of extract will be different. I think, Darwin420 has a good point about EtOH and chlorophyll. BTW for both, it is supposed to be HCl!!! HCL would be some ...

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by JackBean
Sun Dec 20, 2009 1:17 pm
 
Forum: Cell Biology
Topic: Help with my Beetroot Lab
Replies: 4
Views: 3511

Who discovered lipase, pepsin, sucrase and lactase? HELP!

... called sucrase because they are the enzymes involved in the splitting of sucrose into fructose and glucose, which is a process called hydrolysis. (Hydroxide and hydrogen ions join to split them). This may be his homework, but it doesn't seem to be a large page full of questions. You could argue ...

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by g0ld3n88
Sun Dec 13, 2009 2:04 am
 
Forum: General Discussion
Topic: Who discovered lipase, pepsin, sucrase and lactase? HELP!
Replies: 3
Views: 10792

Biochemical Buffers Confusion

first calculate how much negative ions you'd need or hydroxide ions for pH 10, then calculate amount of solute you need based on the dissociation constant.

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by mith
Mon Oct 05, 2009 6:25 pm
 
Forum: Molecular Biology
Topic: Biochemical Buffers Confusion
Replies: 3
Views: 1955
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