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Hydrolysis

Definition

noun

(chemistry)

(1) A chemical reaction in which the interaction of a compound with water results in the decomposition of that compound.

(2) A chemical reaction wherein a water molecule (HOH) and a reactant exchange functional groups resulting in two end products, one containing the hydrogen cation (H) and the other the hydroxyl anion (OH). An example is the chemical reaction of water with the ions of salt resulting in the formation of an acid and a base, one or both of which is only slightly dissociated.

(3) The process of splitting a compound into fragments with the addition of water; a kind of reaction that is used to break down polymers into simpler units, e.g. starch into glucose.


Supplement

Word origin: Gk. hydor = water + lyein = to loosen, dissolve.
Related forms: hydrolytic (adjective), hydrolyze (verb).
See also: water.


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Taqman probe design using FISH probe (need help)

... need to be at least 1 kb long, to hybridize and "highlight" the target chromosomal DNA (metaphase chromosomes or interphase nuclei). Hydrolysis probes (such as Taqman) are very short and used in combination with other two oligonucleotides. the size of the amplicon is very small, usually ...

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by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 534

Re: Why is ATP rather than GTP, CTP or TTP the energy currency?

... forward in terms of energy, and why don't ribosomes also catalyse the reverse reaction of peptide formation, now I guess it's due to the GTP hydrolysis? But I also have some questions about the above post, maybe someone can help me? Firstly, GTP hydrolysis is SLOW .* The extra amount of time ...

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by Maus
Tue Feb 03, 2015 6:46 pm
 
Forum: Molecular Biology
Topic: Why is ATP rather than GTP, CTP or TTP the energy currency?
Replies: 6
Views: 9538

Starch Hydrolysis

Normal RECIPE for STRACH Hydrolysis: Starch agar composition (g/liter) Beef extract 3 g Soluble starch 10 g Agar 12 g Distilled water 1 liter But should we can use Peptone instead of Beef extract ?

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by Helics
Sun Nov 30, 2014 10:00 am
 
Forum: Microbiology
Topic: Starch Hydrolysis
Replies: 0
Views: 544

blank and substrate control give OD reads

... here. Hopefully all of u can solve my problem. I'm working on screening of xylanase variants to look for the better variant than the wild type in hydrolysis kenaf. the reaction is done in 96 deep well plate by adding substrate (kenaf) + buffer (50mM sodium acetate, pH 5.0) + enzyme solution and ...

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by intandiana
Wed Jan 15, 2014 7:21 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
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Views: 6509

Streptococcus sanguis??

... dulcitol: - Nitrate reduction: + Citrate utilisation: - Indole production: - H2S production: - Methyl red test: - Voges Proskauer test: - Urea hydrolysis: - Gelatin hydrolysis: -

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by microbiologyhelp
Fri Dec 20, 2013 10:08 pm
 
Forum: Microbiology
Topic: Streptococcus sanguis??
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