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Dictionary » H » Hybridize

Hybridize

Definition

verb

(biology) To produce or cause to produce a hybrid from crossing parents of different varieties or species; to crossbreed.

(molecular biology) To form a double-stranded nucleic acid from joining two complementary strands of DNA (or RNA).

(chemistry) To form new orbitals suitable for bonding by mixing atomic orbitals.


Supplement

Variant: hybridise.
Related terms: hybrid (noun), hybridization or hybridisation (noun).


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Results from our forum


How to draw a blot from a RFLP physical map?

... thick horizontal line (at top). You cut DNA from different individuals with EcoRI, separate fragments by electrophoresis, blot to a membrane, and hybridize the blot to a labeled probe. Draw a picture of what the blot will look like when the DNA comes from individuals homozygous for the following ...

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by ssimon10
Wed Nov 23, 2011 2:43 pm
 
Forum: Genetics
Topic: How to draw a blot from a RFLP physical map?
Replies: 7
Views: 1711

cloning

... to clone big genes the way is amplify them with PCR from the whole DNA of the organism which carry it. First we design two oligonucleotides wich hybridize with the 5´and 3´ends and carry restriction endonucleases sites to introduce the gene in a subcloning plasmid and after production purification ...

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by protold
Thu May 05, 2011 5:54 am
 
Forum: Molecular Biology
Topic: cloning
Replies: 10
Views: 4129

cDNA hybridization

the cDNA produced using mature mRNA is not exactly complementary to the gene sequence, right? jst wondering how does the cDNA hybridize with the DNA? is it that only part of the cDNA hybridize with the complementary DNA sequence?

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by june320
Fri Oct 29, 2010 1:24 pm
 
Forum: Genetics
Topic: cDNA hybridization
Replies: 3
Views: 1464

Weird questions?

... within this piece of genomic DNA. The genes corresponding to these trasncripts were named P20 and P50. A probe complimentary to 5' end of P20 mRNA hybridized to 0.7kb fragment while the one complimentary to 3' end hybridzied to 0.3kb. Similarly, the probe complimentary to 5' of P50 mRNA hybridize ...

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by yelainec
Mon Mar 01, 2010 4:24 am
 
Forum: Genetics
Topic: Weird questions?
Replies: 1
Views: 928

How to draw a DOT blot from a physical map of RFLP analysis?

... thick horizontal line (at top). You cut DNA from different individuals with EcoRI, separate fragments by electrophoresis, blot to a membrane, and hybridize the blot to a labeled probe. Draw a picture of what the blot will look like when the DNA comes from individuals homozygous for the following ...

See entire post
by supriabio
Fri Nov 27, 2009 4:08 am
 
Forum: Genetics
Topic: How to draw a DOT blot from a physical map of RFLP analysis?
Replies: 3
Views: 2300
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