Dictionary » H » Hybridize

Hybridize

Definition

verb

(biology) To produce or cause to produce a hybrid from crossing parents of different varieties or species; to crossbreed.

(molecular biology) To form a double-stranded nucleic acid from joining two complementary strands of DNA (or RNA).

(chemistry) To form new orbitals suitable for bonding by mixing atomic orbitals.


Supplement

Variant: hybridise.
Related terms: hybrid (noun), hybridization or hybridisation (noun).


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Taqman probe design using FISH probe (need help)

... they are both called "probes", FISH and Taqman probes have different characteristics. Probes for FISH need to be at least 1 kb long, to hybridize and "highlight" the target chromosomal DNA (metaphase chromosomes or interphase nuclei). Hydrolysis probes (such as Taqman) are ...

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by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 543

Is complexity inevitable?

... a very good way of increasing that complexity. If a pair of organisms are in this kind of symbiotic relationship it makes it harder for them to hybridize with other organisms, thus preserving the complexity of the environment. A stable environment throughout its life produces more and more of ...

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by animartco
Sat Aug 24, 2013 11:50 am
 
Forum: Evolution
Topic: Is complexity inevitable?
Replies: 16
Views: 17831

How to draw a blot from a RFLP physical map?

... thick horizontal line (at top). You cut DNA from different individuals with EcoRI, separate fragments by electrophoresis, blot to a membrane, and hybridize the blot to a labeled probe. Draw a picture of what the blot will look like when the DNA comes from individuals homozygous for the following ...

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by ssimon10
Wed Nov 23, 2011 2:43 pm
 
Forum: Genetics
Topic: How to draw a blot from a RFLP physical map?
Replies: 7
Views: 3860

cloning

... to clone big genes the way is amplify them with PCR from the whole DNA of the organism which carry it. First we design two oligonucleotides wich hybridize with the 5´and 3´ends and carry restriction endonucleases sites to introduce the gene in a subcloning plasmid and after production purification ...

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by protold
Thu May 05, 2011 5:54 am
 
Forum: Molecular Biology
Topic: cloning
Replies: 10
Views: 6189

cDNA hybridization

the cDNA produced using mature mRNA is not exactly complementary to the gene sequence, right? jst wondering how does the cDNA hybridize with the DNA? is it that only part of the cDNA hybridize with the complementary DNA sequence?

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by june320
Fri Oct 29, 2010 1:24 pm
 
Forum: Genetics
Topic: cDNA hybridization
Replies: 3
Views: 1831
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