Dictionary » H » Hybridise




(biology) To produce or cause to produce a hybrid from crossing parents of different varieties or species; to crossbreed.

(molecular biology) To form a double-stranded nucleic acid from two complementary strands of DNA (or RNA).

(chemistry) To form new orbitals suitable for bonding by mixing atomic orbitals.


Variant: hybridize.
Related terms: hybrid (noun), hybridization or hybridisation (noun).

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Reading a FISH image

Hey guys, Can someone tell me how to read and interpret a FISH image? If, for example, a FISH test is carried out using probes that hybridise to centromeres 13 and 21, 14 and 22 and 15 and we can see res spots in chromosomes 13 and 21 centromere and does not indicate this in the marker, ...

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by DivyaG
Fri Nov 26, 2010 9:58 pm
Forum: Genetics
Topic: Reading a FISH image
Replies: 0
Views: 855

The Fiber Disease

... enzymes make double stranded breaks, which, if the site of cleavage is off centre, generates fragments with short single stranded tails, these can hybridise to the tails of other fragments and are called sticky ends. They are generally named according to the bacterium from which they were isolated ...

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by al
Sun Dec 24, 2006 4:08 am
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 6245795

The Fiber Disease

... 1984). Since then, two different approaches have evolved. First, the use of exogenous short (15-25 bases), synthetic oligonucleotides, designed to hybridise to specific sequences of target mRNAs via Watson-Crick base pairs and predicted to act mainly by degradation of targeted mRNA by an RNase ...

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by Nadas Moksha
Thu Oct 26, 2006 12:47 am
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 6245795

gel electrophoresis question

... DNA molecules all i can think of is that electrophoresis 1 separates them into different size, shape and charge 2 dna is negatively charged so can hybridise what are the other advantages??

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by kabuto
Mon Aug 21, 2006 1:57 pm
Forum: Molecular Biology
Topic: gel electrophoresis question
Replies: 1
Views: 1159

The Southern Blot technique

... DNA probes locate the fragment of interest because the DNA fragment containg the fragment of interest is double-stranded....so how does the probe hybridise to it? Also why blot the DNA on nitorcellulose filter paper?

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by student12
Wed Jul 05, 2006 9:03 pm
Forum: Genetics
Topic: The Southern Blot technique
Replies: 6
Views: 3587
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