Hybridise

Hey guys, Can someone tell me how to read and interpret a FISH image? If, for example, a FISH test is carried out using probes that hybridise to centromeres 13 and 21, 14 and 22 and 15 and we can see res spots in chromosomes 13 and 21 centromere and does not indicate this in the marker, ...

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... enzymes make double stranded breaks, which, if the site of cleavage is off centre, generates fragments with short single stranded tails, these can hybridise to the tails of other fragments and are called sticky ends. They are generally named according to the bacterium from which they were isolated ...

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... 1984). Since then, two different approaches have evolved. First, the use of exogenous short (15-25 bases), synthetic oligonucleotides, designed to hybridise to specific sequences of target mRNAs via Watson-Crick base pairs and predicted to act mainly by degradation of targeted mRNA by an RNase ...

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... DNA molecules all i can think of is that electrophoresis 1 separates them into different size, shape and charge 2 dna is negatively charged so can hybridise what are the other advantages??

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... DNA probes locate the fragment of interest because the DNA fragment containg the fragment of interest is double-stranded....so how does the probe hybridise to it? Also why blot the DNA on nitorcellulose filter paper?

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