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Dictionary » H » Hybridisation

Hybridisation

Definition

noun

(biology) The act or process of mating organisms of different varieties or species to create a hybrid.

(molecular biology) The process of forming a double-stranded nucleic acid from two complementary strands of DNA (or RNA).

(chemistry) The mixing of atomic orbitals to form new orbitals suitable for bonding.


Supplement

In biology, hybridization (or hybridisation) is usually used as a term in agriculture or in plant production where new hardy and disease-resistant crops are formed.


Variant: hybridization.
Related terms: hybrid (noun), hybridize or hybridise (verb).


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Re: Issues of EST in whole genome gene discovery

... achieve. It would also have the advantage of providing a picture as to which spliced variants occur. High throughput normalisation (subtractive hybridisation would eliminate 90% of the known housekeeping as well (or DNA chip screening, but you do miss the advantage of the splice variants). 2. ...

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by merv
Sun Oct 02, 2011 7:46 pm
 
Forum: Bioinformatics
Topic: Issues of EST in whole genome gene discovery
Replies: 3
Views: 3269

Design to detect transcription factor specificity

Thanks, thats brilliant. I was looking into the hybridisation with the tagged TF which seems like it would work. Also with the chip will it be appropriate for bacteria? I just got a little confused as bacteria do not have chromatin? Thanks

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by sam2400
Mon Jun 27, 2011 3:54 am
 
Forum: Microbiology
Topic: Design to detect transcription factor specificity
Replies: 2
Views: 245

Improving hybridisation signal by removing cytoplasm

Hello everybody over the years we have had persistent & frustrating problems with cytoplasm and its affect on hybridisation FISH probes. Its become clear to me that pre-treatment to remove cytoplasm from samples is key to enable efficient and successful hybridisation of the ...

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by jamesthe3
Fri Oct 29, 2010 2:54 am
 
Forum: Molecular Biology
Topic: Improving hybridisation signal by removing cytoplasm
Replies: 1
Views: 640

Fc fusion probes

Hi I was wondering if anyone could tell me how Fc/AP fusion probes work? Can you use them as you would in a normal in situ hybridisation to see expression patterns? Also what are the benefits to using them? I've tried searching the net for this but there seems to be a real lack of basic ...

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by sophie1983
Thu Nov 19, 2009 9:42 am
 
Forum: Molecular Biology
Topic: Fc fusion probes
Replies: 3
Views: 448

PCR

... the Bg/II and HindIII sites. b) A radio-labeled gene x probe is added to the Southern blots that contain the DNA. Resulting autoradiograph showed hybridisation with 3.7 kb Bg/II and 11.8 kb HindIII fragments. Indicate the location of gene x on the physical map of the DNA. Thanks :)

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by Missi
Wed Nov 04, 2009 5:51 am
 
Forum: Molecular Biology
Topic: PCR
Replies: 2
Views: 448
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