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Housekeeping gene


noun, plural: housekeeping genes

Any of the genes that are constitutively expressed at a relatively constant level across many or all known conditions.


Housekeeping genes are those genes that are always expressed because they code for proteins that are constantly required by the cell, hence, they are essential to a cell and always present under any conditions. It is assumed that their expression is unaffected by experimental conditions. The proteins they code are generally involved in the basic functions necessary for the sustenance or maintenance of the cell. Examples of housekeeping genes include actin, GAPDH and ubiquitin.

Compare: luxury gene.
See also: genetic expression, transcription.

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Why must we avert the genome of Eden?

... of the LUA, which was revealed by quite recent discoveries in genetics. Contrary to earlier expectations the distribution of genes amongst ... interpret this failure supposed to be, that these similarities reflect housekeeping genes. This interpretation is unsustainable in the light of ...

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by Hunor
Mon Sep 23, 2013 12:08 am
Forum: Evolution
Topic: Why must we avert the genome of Eden?
Replies: 6
Views: 14528

Problem with the normalization of PCR Ct values

... that one way for normalizing PCR data is to divide the Ct of the gene of interest by the Ct obtained from a housekeeping/reference gene (Ct inter. / Ct refer.). However, I am not sure how to interpret ...

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by Tzia
Tue Apr 30, 2013 12:41 pm
Forum: Molecular Biology
Topic: Problem with the normalization of PCR Ct values
Replies: 5
Views: 8289

Question regarding about PCR - DNA or RNA as template

... I obtained very interesting results which corresponding bands of housekeeping gene were detected when RNA was used as template but not the target gene. We thought of possibly ...

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by Xenotoxic
Fri Aug 17, 2012 3:55 am
Forum: Molecular Biology
Topic: Question regarding about PCR - DNA or RNA as template
Replies: 1
Views: 4333

PCR from cDNA question

Hi All, I want to PCR a 2kb long segment of a gene out of the cDNA made from total RNA extract but have been experiencing many ... factors highly expressed at the same time, no bands at all. for a housekeeping gene in the species (ubiquitin), no bands. the regions of these gene ...

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by vastgenome
Fri Jun 22, 2012 3:55 pm
Forum: Molecular Biology
Topic: PCR from cDNA question
Replies: 1
Views: 4085

Primer design

... of the amplified sequence. with the following criteria to be noted : housekeeping gene has to be amplified at the same time, same sample etc, target mRNA should produce a DNA ...

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by NathanKAPOW
Fri Dec 16, 2011 1:02 am
Forum: Molecular Biology
Topic: Primer design
Replies: 2
Views: 1786
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