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Housekeeping

Housekeeping

1. The state of being occupying a dwelling house as a householder.

2. Care of domestic concerns; management of a house and home affairs.

3. Hospitality; a liberal and hospitable table; a supply of provisions. Tell me, softly and hastly, what's in the pantry? Small housekeeping enough, said phoebe. (Sir W. Scott)


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Why must we avert the genome of Eden?

... hoc interpretations presented after the data arrives. However, one way to interpret this failure supposed to be, that these similarities reflect housekeeping genes. This interpretation is unsustainable in the light of the more recent findings. Richard Dawkins in his book “The Blind Watchmaker” ...

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by Hunor
Mon Sep 23, 2013 12:08 am
 
Forum: Evolution
Topic: Why must we avert the genome of Eden?
Replies: 6
Views: 7521

Problem with the normalization of PCR Ct values

... simultaneously. I have read somewhere that one way for normalizing PCR data is to divide the Ct of the gene of interest by the Ct obtained from a housekeeping/reference gene (Ct inter. / Ct refer.). However, I am not sure how to interpret the resulting values. Is it true that the larger the ratio, ...

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by Tzia
Tue Apr 30, 2013 12:41 pm
 
Forum: Molecular Biology
Topic: Problem with the normalization of PCR Ct values
Replies: 5
Views: 3668

Question regarding about PCR - DNA or RNA as template

... is that I did PCR with RNA and cDNA simultaneously and did gel electrophroesis. I obtained very interesting results which corresponding bands of housekeeping gene were detected when RNA was used as template but not the target gene. We thought of possibly of genomic DNA contamination but when ...

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by Xenotoxic
Fri Aug 17, 2012 3:55 am
 
Forum: Molecular Biology
Topic: Question regarding about PCR - DNA or RNA as template
Replies: 1
Views: 3694

PCR from cDNA question

... gene products: for some proteases, very bright bands. for some transcription factors highly expressed at the same time, no bands at all. for a housekeeping gene in the species (ubiquitin), no bands. the regions of these gene i am amplifying are all 2kb, cycler conditions and PCR rxn mix followed ...

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by vastgenome
Fri Jun 22, 2012 3:55 pm
 
Forum: Molecular Biology
Topic: PCR from cDNA question
Replies: 1
Views: 3133

Primer design

... came up with a set of primers. Now i have to calculate the approximate length of the amplified sequence. with the following criteria to be noted : housekeeping gene has to be amplified at the same time, same sample etc, target mRNA should produce a DNA distingushable with housekepper and taq polymerase ...

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by NathanKAPOW
Fri Dec 16, 2011 1:02 am
 
Forum: Molecular Biology
Topic: Primer design
Replies: 2
Views: 1560
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