Housekeeping

... (albeit more ambiguous and longer) way and try to identify specific genes within your sequence's DNA. For example, try to compare it to a human housekeeping gene such as GapDH or HSP70. If it doesn't contain it, then it is most likely not a human cell. And so on.

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Mr Mistery is right: -RTqPCR if you have a few genes. Depending on the machines you can multiplex 3 to 5 genes (and that would include a control housekeeping gene to normalize for variations in RT efficiency). The positive side is that you can work with a truckload of samples (say 40 in a 96 wells ...

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... intensity of the signal depends on the number of attached molecules. you can read deduce the relative expression of a gene compared to a standard (housekeeping gene usually) just as well as with qPCR.

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Does anyone know something about housekeeping genes frequently used with Danio rerio? I actually use GAPDH and beta actin for Q-PCR, and I'm searching a third gene to confirm my results.

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... were targeted by soaking adult female B. malayi in dsRNA complementary to these transcripts in the optimised culture system. Targeting of the two housekeeping genes Bm-tub-1 and Bm-ama-1 led to a reduction in the levels of their transcripts, as assessed by reverse transcriptase coupled PCR (RT-PCR), ...

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