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Horseradish peroxidase

Horseradish peroxidase

(Science: enzyme) a large enzyme, frequently used in conjunction with diaminobenzidine as an intracellular marker to identify cells both at light and electron microscopic levels.


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Re: Difference between ICC and IF

... primary antibody and anti-goat-IgG secondary antibody conjugated to horseradish peroxidase.

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by jonmoulton
Mon Feb 25, 2013 5:11 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 17525

Molecular Cell Biology

... cell line with epidermal growth factor (EGF) to measure RME, and horseradish peroxidase (HRP) to measure FPE. A quantitative analysis was carried out by incubating A431 ...

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by Freckles
Wed Dec 07, 2011 11:24 pm
 
Forum: Cell Biology
Topic: Molecular Cell Biology
Replies: 5
Views: 4257

Colony staining and counting of bifidobacteria bb-12

... not entirely knowledgeable in this field, but will it work if you use horseradish peroxidase as the growth indicator, as it gives color when oxidized by hydrogen peroxide? You ...

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by aptitude
Sun Sep 11, 2011 6:57 am
 
Forum: Microbiology
Topic: Colony staining and counting of bifidobacteria bb-12
Replies: 1
Views: 3617

Horseradish Peroxidase protein does not SHow up on PAGE gel

Okay, i ran HRP with polyacrylamide gel electrophoresis, the buffer that i used to store the HRP is SOdium phosphate monohydrate and it worked out alright. I loaded the wells with beta-mercaptoethanol and all the proteins had sodium dodecyl sulphate (SDS), all my proteins ran succesfuly on the gel e...

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by AlaRoshdieh
Thu May 20, 2010 2:50 am
 
Forum: Molecular Biology
Topic: Horseradish Peroxidase protein does not SHow up on PAGE gel
Replies: 2
Views: 1968

Re: blood brain barrier

... brain. Using electron microscopy and electron- dense tracers such as horseradish peroxidase (HRP) a group of scientists demonstrated that the blood- brain barrier is located ...

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by lipoflorida
Fri Jun 19, 2009 10:05 am
 
Forum: Physiology
Topic: blood brain barrier
Replies: 2
Views: 11058
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