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Homogenate

Definition

noun, plural: homogenates

Material that has been homogenized or obtained through homogenization.


Supplement

For example, a liver homogenate is a liver tissue in which its cells have been mechanically disrupted, releasing the organelles and cytoplasm.

The word homogenate was introduced by VR Potter in1941 (J. Biol. Chem 141:775) to refer specifically to suspensions or animal tissues that had been ground in the all-glass "homogenizer"as described by Potter and CA Elvehjem in 1936 (J. Biol. Chem114:495) In the inaugural volume of Methods in Medical Reseach (Vol 1, Van R. Potter, editor-in-chief Yearbook Publishers Chicago:1948) Potter notes that the term had since been used by various investigators to refer to tissue preparations that have been "ground in mortor, with or without sand, or disintegrated in a Waring blendor, or produced by methods not described." (p. 317) Although these preparations are probably no less appropriately called homogenates, Potter says, in line with the stated goals of Methods in Medical Research, "it seems desirable to promote a nomenclature that is as meaningful as possible, and it is suggested that the method of preparation be specified." (p.317) The chief significance of the term, he continues, is that "it serves to distinguish the preparation from slices, minces and extracts....the homogenate technique accepts the fact that the cells in the tissue are no longer living and attempts to obtain surviving groups of enzymes [word "enzymes" emphasized] without loss of in vivo properties."


Related forms: homogenize (verb), homogenization (noun).


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Calculating CFU/g

... example the following colony counts in dilution 10-3: Dilution Replicate (1) Replicate (2) 10-3 315 306 I know how to calculate the CFU/cm3 of the homogenate. But, how do we calculate the average CFU/g of food sample? Thanks.

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by SWEngineer
Fri Nov 25, 2011 10:29 am
 
Forum: Microbiology
Topic: Calculating CFU/g
Replies: 2
Views: 7092

Re: Biology Lab Help - Protein Assays

... if you are working with a purified protein sample. However, very often you would like to measure the total protein concentration of a lysate, a homogenate or any other preparation in which there is a mixture of components. Any UV-absorbing components will add to the background, and these preparations ...

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by clevermizo
Wed Feb 03, 2010 5:37 pm
 
Forum: Molecular Biology
Topic: Biology Lab Help - Protein Assays
Replies: 4
Views: 4472

Food Microbiology - Escherichia coli O157:H7 in Meat

... for most of this kind of organisms, samples should be 25g and the diluent peptone water (225ml). But that was a long time ago. I think for your homogenate, 1g might be a little too small a sample. For O157:H7 no need for blood agar, Nutrient agar is not selective so not good. SMAC is used for ...

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by canalon
Tue Jan 13, 2009 4:23 am
 
Forum: Microbiology
Topic: Food Microbiology - Escherichia coli O157:H7 in Meat
Replies: 3
Views: 3961

Food Microbiology - Escherichia coli O157:H7 in Meat

... concerning food microbiology, and I need answers for the following: I am detecting Escherichia coli O157:H7: in lamb meat.I will grow the sample homogenates on Sorbitol MacConkey Agar! For preparing the sample homogenate, what is the preferable diluent to be used? Why ?Is it sterile water, MRD, ...

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by kady123
Sun Jan 11, 2009 8:47 pm
 
Forum: Microbiology
Topic: Food Microbiology - Escherichia coli O157:H7 in Meat
Replies: 3
Views: 3961

Re: Question help

ahh ok, thx!... i hv a few more that i need help with: what are the advantages and disadvantages of autoradiography or immunohistochemistry over homogenate binding? ...disadvantages of homogenate binding will include non-specific binding and also there has to be a radioactive form of the ligand ...

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by biology_06er
Sat Jun 21, 2008 4:18 am
 
Forum: General Discussion
Topic: Question help
Replies: 4
Views: 2273
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