Dictionary » H » Holoenzyme

Holoenzyme

A conjugated enzyme, meaning that it possesses a biological living part and a non-biological, non-living part. See the protein variety page in the cell biology tutorial for related information.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


3' to 5' Exonuclease question

... the 3' to 5' exonuclease and polymerase" (probably polymerase III). What does that mean? How can it be a race since the DNA Polymerase is a holoenzyme/molecular machine/replisome which pretty much means that all the subunits are connected? That's like if I were to tie a string onto two matchbox ...

See entire post
by NathanielZhu
Thu Jul 07, 2011 2:03 am
 
Forum: Molecular Biology
Topic: 3' to 5' Exonuclease question
Replies: 2
Views: 3096

gene expression and alternative sigma factors

... activation of different regulons, and this is used in the many stress responses (e.g. of e.coli) But, on the genome, can operons induced by the holoenzyme incorporating one sigma factor overlap those that are activated as the result of another sigma factor? If not, why not, and if yes, then ...

See entire post
by neon
Tue Jun 13, 2006 9:20 am
 
Forum: Molecular Biology
Topic: gene expression and alternative sigma factors
Replies: 0
Views: 1101

Multiple proteins encoded in single operon

... the end of one protein coding region and another, then what is responsible for the termination of the transcription process - i.e. how would the holoenzyme know when to stop transcribing the DNA? Thanks very much in advance! Neon

See entire post
by neon
Fri Jun 09, 2006 9:07 am
 
Forum: Molecular Biology
Topic: Multiple proteins encoded in single operon
Replies: 1
Views: 1426

Define:Transport pathways. Details on sigma factors

... in an operon (operonA), then this requires (amongst other things) RNAP and a sigma factor - call this sfA. When sfA and RNAP bind and form the holoenzyme, the genes in operonA are transcribed. (ignore any activators/repressors for now). OperonB contains promB etc...If only sfA is present, the ...

See entire post
by neon
Mon May 29, 2006 12:09 pm
 
Forum: Molecular Biology
Topic: Define:Transport pathways. Details on sigma factors
Replies: 1
Views: 1615

The Idea

... ori, the leading strand will be leading throught its replication and the lagging will be lagging. The replication enzyme being employed is not the holoenzyme E.Coli PolI but its Klenow fragment. A Klenow fragment lacks 'nick-translation' activity. Therefore the RNA primers are not degraded. Hence ...

See entire post
by cytochromeP
Tue Aug 23, 2005 7:10 am
 
Forum: Molecular Biology
Topic: Centrifugation query
Replies: 13
Views: 7236
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 9,551 times. 
What links here | Related changes | Permanent link