Dictionary » H » Histidine



(Science: amino acid) An amino acid with an imidazole side chain with a pKa of 6-7. Acts as a proton donor or acceptor and has high potential reactivity and diversity of chemical function. Forms part of the catalytic site of many enzymes. An essential amino acid found in proteins that is important for the growth and repair of tissue.One of twenty amino acids commonly found in [[proteins.

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Why dialyse DMEM before protein purification (Ni-Affinity)?

... secreted. I guess you have removed the cells and probably concentrated the medium and exchanged with some buffer, right? The medium contains some histidine, but at low concentration (0.2 mM). More important will be probably pH of the medium, because it is not buffered.

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by JackBean
Sun May 20, 2012 8:16 pm
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 4020

Re: how to memorize the amino acid

... you just add benzene to the Ala and you have (surprisingly :lol:) phenylalanine; just add hydroxyl and you get tyrosine. Add imidazole and you get histidine. With the largest system you get tryptophan ;) For the acidic AAs, just add carboxyl to Ala and you get aspartic acid. Since G lutamic acid ...

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by JackBean
Thu Feb 09, 2012 9:52 am
Forum: General Discussion
Topic: how to memorize the amino acid
Replies: 4
Views: 7185

Edman Degradation Problem

... goes over which amino acids can be phosphorylated...but after some research on the internet I've come up with: serine, threonine, tyrosine, and histidine? Is this right? And as for the enzyme... either kinase or phosphatase? F) Again, I think I need the amino acid code for this, but I know that ...

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by pinkerpikachu
Sun Oct 16, 2011 7:35 pm
Forum: Molecular Biology
Topic: Edman Degradation Problem
Replies: 1
Views: 5401

medium choice confusion

... Genotype Phenotype thi - Thi- cannot synthesise vitamin B1 (thiamine) trp - Trp- cannot synthesise tryptophan his - His- cannot synthesise histidine met - Met- cannot synthesise methionine arg - Arg- cannot synthesise arginine thr - Thr- cannot synthesise threonine phe - Phe- cannot synthesise ...

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by ashhh
Sun Aug 21, 2011 2:58 pm
Forum: Molecular Biology
Topic: medium choice confusion
Replies: 1
Views: 3586

Properties of Amino Acid Side Chains

... web site I go to says something different, and my professor didn't add that to his slides. I know that Aspartic and Glutamic Acid are acidic; that Histidine, Arginine and Lysine are basic; but as for the other properties I mentioned...? Thanks, Holly

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by NiceOnTheIce
Thu Nov 04, 2010 11:28 pm
Forum: Molecular Biology
Topic: Properties of Amino Acid Side Chains
Replies: 1
Views: 2684
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