His operon

How can gene regulation and expression be manipulated for designing expression vectors? And as a result, what are their potential biotechnological applications?

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I am trying to incorporate bioinformatics regarding the lac operon. Any ideas as to how? I'm looking at the e. coli cells changing from a glucose medium to a lactose medium?

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... bacteria. We had been able to generate a single mutant using this method before, but we're having trouble deleting a gene within the same region (operon), and the issue (and difference between the two) are not so obvious for us. Our vector is a circular plasmid. Can you elaborate on what you mean ...

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... the following: a)C ------------ 1)U,C or A b)A ------------- 2)G c)U ------------ 3)C or U d)G ------------ 4)U e) I ------------ 5)A or G 5) Lac operon= Imagine that in a e.coli culture that was growing with glucose as its nutrient source you could artificially increase cAMP levels (which are ...

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... DNA sequence region containing the UAS sites upstream of GAL2 is deleted in this mutant. You are studying the genetics of a negatively regulated operon in E. coli, in which a single mRNA transcript encodes two genes (A and B) that are separately translated into two proteins A and B. You have ...

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