Heterozygosity

sorry let me rephrase my question how exactly is the assembly done ? I am asking this because how specifically do they detect heterozygosity ?

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... signals. Background noise, however, is common. If your sequence is generally clean and you come across sites as in your examples, I would suspect heterozygosity at both sites. Ideally, it is best to have known homozygotes as controls for interpreting such data.

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... that just be (total # of alleles)/11 calculated for each column? The allele frequencies should just be read off the table, I think. Then the %heterozygosity for each locus can be calculated per taxon with Hardy-Weinberg, as above, and the average heterozygosity would be the average of the ...

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I’m not at all sure I’m doing this correctly—I’m not a geneticist by trade. It seems to me that the average heterozygosity is zero for douglasii (100% homozygous for TfC) and S. parryii (100% homozygous for TfA)—unless you have to take into account the possibilty of a sampling ...

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... taxon (%P) 2) Average number of alleles per loci 3) For each locus within each taxon, calculate allele frequency of each allele at each locus. 4) Heterozygosity at each locus for each taxon. 5) Average heterozygosity per taxon. http://answerboard.cramster.com/answer-board/image/637972a7455c94cd383e791d0167ed12.jpg ...

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