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Heparin

Heparin

(Science: drug) Sulphated mucopolysaccharide, found in granules of mast cells, that inhibits the action of thrombin on fibrinogen by potentiating antithrombins, thereby interfering with the blood clotting cascade. Platelet factor iv will neutralise heparin.


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blood

... or metabolites that are extremely unstable for one reason or another. You can add things like citrate as a preservative, I think, but not EDTA or heparin; if you inhibit the clotting system you will be separating out plasma, not serum.

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by blcr11
Tue Nov 27, 2007 4:17 pm
 
Forum: Molecular Biology
Topic: blood
Replies: 5
Views: 648

Cell passage

yes yes you are right, we add FBS PS and heparin as well as ECGS instead to buy complete media.... good luck in anyways....I am glad to help you...

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by konstantin
Thu Jun 07, 2007 5:01 am
 
Forum: Cell Biology
Topic: Cell passage
Replies: 6
Views: 2370

Cell passage

... study how well EC adhere to the substrate...I would like to find out more about ur work....ur using 1% of gelatin, and do u mean u add in FBS, heparin, ECGS, and PS? Instead of buying a complete medium? Thanks for ur help!

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by Endothelial-cell
Thu Jun 07, 2007 1:20 am
 
Forum: Cell Biology
Topic: Cell passage
Replies: 6
Views: 2370

Cell passage

... our lab..use HYQ 199/EBSS medium from HyClone....as well as content of medium is a little different for example instead of 10% I use 20% of FBS, heparin, ECGS, and PS..... so I hope this information will be useful for you if you have additional questions don't hesitate

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by konstantin
Fri Jun 01, 2007 3:54 am
 
Forum: Cell Biology
Topic: Cell passage
Replies: 6
Views: 2370

pls help- URGENT!!!

... effect of the toxins. They help to kill bacteria too. For the clotting of blood, it does not happen normally due to an anti-clotting substance heparin which is produced in the liver. When thrombokinase is released, it neutralises the action of heparin so that the clotting takes place. Serum ...

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by unscientific
Tue Mar 06, 2007 2:06 pm
 
Forum: Human Biology
Topic: pls help- URGENT!!!
Replies: 4
Views: 1021
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