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Heparin

Heparin

(Science: drug) Sulphated mucopolysaccharide, found in granules of mast cells, that inhibits the action of thrombin on fibrinogen by potentiating antithrombins, thereby interfering with the blood clotting cascade. Platelet factor iv will neutralise heparin.


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centrifugation of hemocytes

I'm not expert in animal biology, but I'd say you do not need EDTA or heparin for resuspension, since the cells are pelleted because of centrifugation, not because they grew there. What about trying some Western or other protein detection after the resuspension ...

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by JackBean
Thu Jan 30, 2014 9:34 am
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 662

centrifugation of hemocytes

... "pelleted" to the bottom of the wells and so when i wash, the cells should stay sticked, unless i resuspend with PBS containing EDTA or heparin... Is it correct? When i take off the medium after centrifugation, and then add PBS are the cell straightforward resuspended or can I take the ...

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by Thom
Tue Jan 28, 2014 10:50 am
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 662

Question about the coagulation cascade

... authoritative and I suspect it is a well intentioned (but incorrect) attempt to describe the process. Also, if anybody can explain to me why heparin inhibits the conversion of XII and XI to the active forms, that would be helpful. I understand that heparin increases the binding affinity of ...

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by Hodag
Sun Jan 03, 2010 12:26 am
 
Forum: Molecular Biology
Topic: Question about the coagulation cascade
Replies: 0
Views: 1226

blood

... or metabolites that are extremely unstable for one reason or another. You can add things like citrate as a preservative, I think, but not EDTA or heparin; if you inhibit the clotting system you will be separating out plasma, not serum.

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by blcr11
Tue Nov 27, 2007 4:17 pm
 
Forum: Molecular Biology
Topic: blood
Replies: 5
Views: 2558

Cell passage

yes yes you are right, we add FBS PS and heparin as well as ECGS instead to buy complete media.... good luck in anyways....I am glad to help you...

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by konstantin
Thu Jun 07, 2007 5:01 am
 
Forum: Cell Biology
Topic: Cell passage
Replies: 6
Views: 4767
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