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Helicase

Helicase --> dna helicase

(Science: enzyme molecular biology) a prokaryote enzyme that uses the hydrolysis of atp to unwind the dna helix at the replication fork, to allow the resulting single strands to be copied.

two molecules of atp are required for each unwinding of the duplex.The enzyme that is capable of unwinding the double helix structure of dna, which alters the hydrogen bonds present in dna. Helicase therefore plays a part in dna replication and also requires some atp to function as activation energy.


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How does a plasmid work?

Can you (or anybody else) go into a little more detail about how the replication works? Or link me to a website? Is it still the same with helicase unwinding the DNA and primase coming in and etc. like in regular DNA replication?

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by WizardofScience
Sun Dec 30, 2012 2:06 pm
 
Forum: Molecular Biology
Topic: How does a plasmid work?
Replies: 2
Views: 1969

Questions on the Sodium Potassium Pump and Enzymes

... For example DNA Ligase forms phosphodiester bonds between Okazaki fragments... What about when one job involves two enzymes ie. DNA Gyrase and DNA Helicase in unwinding the double helix or when an enzyme has two jobs such as DNA Polymerase forming phosphodiester bonds between nucleotides and adding ...

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by JoWillyTso
Mon Nov 05, 2012 5:50 pm
 
Forum: Cell Biology
Topic: Questions on the Sodium Potassium Pump and Enzymes
Replies: 3
Views: 3789

Scientific paper on DNA replication & gel electrophoresis

... This suggests that the oriC-loaded replisome can catalyze their formation without the need to recruit additional DNA polymerase or helicase. What I don't understand: 1. Why are the stalled forks about 23 kbp while the full length DNA are only 9.4 kbp (on figure 1)? 2. Did they prepare ...

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by dekk1525
Sat Sep 08, 2012 11:17 pm
 
Forum: Molecular Biology
Topic: Scientific paper on DNA replication & gel electrophoresis
Replies: 0
Views: 4923

Re: Do the simplest bacteria have ribosomes and helicase?

This is not directly addressing the questions in this discussion, but the topic is closely related and might be of interest. http://blogs.nature.com/news/2012/06/dissolved-iron-may-have-been-key-to-rna-based-life.html#wpn-more-19146 I am including it here as an example of the ongoing...

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by jonmoulton
Fri Jun 01, 2012 3:10 pm
 
Forum: Molecular Biology
Topic: Do the simplest bacteria have ribosomes and helicase?
Replies: 27
Views: 13993

Re: Do the simplest bacteria have ribosomes and helicase?

Some chemical reactions were likely producing biochemical precursors and replicating the molecules needed to catalyze the precursor-building reactions prior to their capture within a cell membrane. These reactions that build the parts of the system which catalyzes the reactions are termed a hypercyc...

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by jonmoulton
Wed May 23, 2012 9:00 pm
 
Forum: Molecular Biology
Topic: Do the simplest bacteria have ribosomes and helicase?
Replies: 27
Views: 13993
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