Heat-stable enzyme

... would be pretty useless. However, as power passes through, heat is released, which could kill your enzyme.

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... 3. gel extraction of insert of interest 4. restriction reaction 5. enzyme removal <- I often use KpnI/BamHI and can't go with heat inactivation 6. ligation with backbone and transformation I know that restriction can ...

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... are basically the same exergonic reaction. However the amount of heat released by fire would destroy a cell. Explain how a cell manages to ... the evidence that supports this. (5) 7. It is often said that the enzyme ATP synthase works like a water wheel turbine. Explain the details ...

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... bond i.e., "Hydrogen bond". This bond can easily disturb by heat, as compare to DNA backbone. In the phosphodiester bond has phosphate ... disturb because presence of phosphodiester bond. Various restriction enzyme cut DNA backbone at specific sites.

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Sure. Say if I don't put the enzyme in at first, just heat the RNA to unfold the secondary structure, what temperature should I use to best unfold the secondary ...

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