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Heat-stable enzyme

Heat-stable enzyme --> thermostable enzyme

(Science: enzyme) An enzyme that is not readily subject to destruction or alteration by heat.

Synonym: heat-stable enzyme.


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can you denature enzymes using electricity?

... would be pretty useless. However, as power passes through, heat is released, which could kill your enzyme.

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by JackBean
Thu Nov 29, 2012 4:20 pm
 
Forum: General Discussion
Topic: can you denature enzymes using electricity?
Replies: 2
Views: 371

restriction reaction on agarose gel slices

... 3. gel extraction of insert of interest 4. restriction reaction 5. enzyme removal <- I often use KpnI/BamHI and can't go with heat inactivation 6. ligation with backbone and transformation I know that restriction can ...

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by dustman
Fri May 25, 2012 2:54 pm
 
Forum: Molecular Biology
Topic: restriction reaction on agarose gel slices
Replies: 2
Views: 871

Cellular Energy Questions

... are basically the same exergonic reaction. However the amount of heat released by fire would destroy a cell. Explain how a cell manages to ... the evidence that supports this. (5) 7. It is often said that the enzyme ATP synthase works like a water wheel turbine. Explain the details ...

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by flower555
Mon Nov 29, 2010 2:05 am
 
Forum: General Discussion
Topic: Cellular Energy Questions
Replies: 2
Views: 5337

Re: DNA backbone breakage

... bond i.e., "Hydrogen bond". This bond can easily disturb by heat, as compare to DNA backbone. In the phosphodiester bond has phosphate ... disturb because presence of phosphodiester bond. Various restriction enzyme cut DNA backbone at specific sites.

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by kanagasundar
Tue Oct 05, 2010 3:54 am
 
Forum: Genetics
Topic: DNA backbone breakage
Replies: 5
Views: 5448

Re: The right temperature for unfolding RNA secondary structure

Sure. Say if I don't put the enzyme in at first, just heat the RNA to unfold the secondary structure, what temperature should I use to best unfold the secondary ...

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by frankalpha
Thu Sep 23, 2010 2:33 pm
 
Forum: Molecular Biology
Topic: The right temperature for unfolding RNA secondary structure
Replies: 3
Views: 1301
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