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Heat-shock protein 27 kinase

Heat-shock protein 27 kinase

(Science: enzyme) Phosphorylates hsp 26 on serine residues when stimulated by tumour necrosis factor or interleukin 1

registry number: EC 2.7.1.-

Synonym: hsp 27 kinase, heat-shock protein 27 kinase, hsp27 kinase

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Re: problem with cloning/restriction digest

... CIP can be partly inactivated by heat, but it is better to phenol extract after CIPing ... days by LIC, which doesn’t require using CIP/Kinase or REs. 2) You don’t have to gel purify the ... heard about are issues with the expression of protein, not with the cloning.

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by blcr11
Fri Feb 22, 2008 11:40 pm
Forum: Molecular Biology
Topic: problem with cloning/restriction digest
Replies: 35
Views: 41473

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