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Harvest

Harvest

1. The gathering of a crop of any kind; the ingathering of the crops; also, the season of gathering grain and fruits, late summer or early autumn. Seedtime and harvest . . . Shall not cease. (gen viii. 22) At harvest, when corn is ripe. (Tyndale)

2. That which is reaped or ready to be reaped or gathed; a crop, as of grain (wheat, maize, etc), or fruit. Put ye in the sickle, for the harvest is ripe. (Joel III. 13) To glean the broken ears after the man That the main harvest reaps. (Shak)

3. The product or result of any exertion or labour; gain; reward. The popes principal harvest was in the jubilee. (Fuller) The harvest of a quiet eye. (Wordsworth) harvest fish see daddy longlegs.

Origin: oe. Harvest, hervest, as. Haerfest autumn; akin to LG. Harfst, D. Herfst, OHG. Herbist, g. Herbst, and prob. To L. Carpere to pluck, gr. Fruit.


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some bio multiple choice questions

1. Winter wheat can be planted in the spring for fall harvest if it is a. sprayed with gibberellins. b. soaked in water. d. planted under a full moon. d. stored in the dark for 50 days. e. vernalized. Answer said it was E. but can't A also be right? ...

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by jjkwest1
Fri Feb 10, 2012 8:00 am
 
Forum: Botany Discussion
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Learning tissue culture, specifically moDC generation

... Then add 25 ug/ml of poly-I:C and incubate another 24 hours, and voilá! With some luck, you have just generated dendritic cells! You can the harvest them with a cell scraper if you so wish, but if you intend to stimulate other cells with them, maybe just leave them on the plate and add the ...

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by biohazard
Fri Feb 03, 2012 2:09 pm
 
Forum: Cell Biology
Topic: Learning tissue culture, specifically moDC generation
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Re:

... no problems here. - Speed will vary in function of equipment available (tubes and centrifuge), so I cannot really comment here - The point is to harvest as much as possible, with my method, you might lose some time, but probably not many more bacteria than any other validated method, and time ...

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by canalon
Mon Dec 12, 2011 4:08 am
 
Forum: Microbiology
Topic: Centrifuging low amount of bacteria
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Views: 1861

Re: Western Blot TroubleShooting

I harvest my samples using RIPA buffer + protease inhibitor on ice. I use a 6 well dish and I scrape the samples off on ice. I rotate the samples at 4C for 1 hour for lysis. I then centrifuge for 30 min. at 13,000 rpm at 4C. ...

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by mutant11
Mon Nov 21, 2011 3:11 am
 
Forum: Molecular Biology
Topic: Western Blot TroubleShooting
Replies: 7
Views: 1687

Western Blot TroubleShooting

... that is expressed at pretty low levels endogenously in cell lines. Does anyone have any suggestions? -I use RIPA buffer with protease inhibitor to harvest the protein. I keep everything on ice. -I incubate in primary antibody + block overnight. Thanks!

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by mutant11
Fri Nov 18, 2011 10:17 pm
 
Forum: Molecular Biology
Topic: Western Blot TroubleShooting
Replies: 7
Views: 1687
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