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Hanes

Hanes plot

a graphical representation of enzyme kinetic data in which the substrate concentration divided by the velocity (i.e., the [S]/v ratio) is plotted on the vertical axis as a function of [S]. Sometimes referred to as the hanes-Wilkinson plot.


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IC50

... either use simple v (speed of reaction) on [S] (as Michaelis-Menten graph), or Lineweaver-Burg (1/v on 1/[S]) or plenty of others (e.g. Dixon's, Hanes-Wolf, Wolf-Augustinson-Hoffsteeh, Hunter-Dowson etc)

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by JackBean
Thu Dec 02, 2010 8:43 pm
 
Forum: Molecular Biology
Topic: IC50
Replies: 2
Views: 1481

The Fiber Disease

... decreased from 12.94/min to 2.93/min and 4.28/min for Neu5Ac2en and pNPO, respectively, when Mu-Neu5Ac was used as substrate. [Figure] Fig. 3. Hanes Wolf plots of initial velocity data of the inhibition of E. histolytica SD-catalyzed hydrolysis of methylumbelliferyl-Neu5Ac (4-MU-Neu5Ac) in ...

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by Nadas Moksha
Sun Dec 10, 2006 3:16 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5493465

Re: Eadie-Hofstee plot

... that the same reasoning is valid to derive the so called Scatchard or Eadie-Hofstee-plot (B/L against B). Another method you didn't mention is the Hanes-plot (L/B against L)

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by sdekivit
Tue Aug 15, 2006 3:06 pm
 
Forum: Molecular Biology
Topic: Eadie-Hofstee plot
Replies: 4
Views: 12469


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