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Dictionary » H » Hairpin loop

Hairpin loop

Hairpin loop

An area where single-stranded dna or rna has folded back on itself and nucleotides from the two separate segments have base paired, so that the resulting structure appears as the name describes.


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Reasons for Skepticism on the Endosymbiosis?

... mechanisms to "prevent" shortening exist, ranging from hairpin loops and self-priming to protein-assisted primer synthesis (see here ). The telomeric regions ...

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by SuperfluousAvatar
Thu Jan 12, 2012 7:43 pm
 
Forum: Cell Biology
Topic: Reasons for Skepticism on the Endosymbiosis?
Replies: 0
Views: 4820

primer designing

... one. Length should be about 19 to 30nt, CG% about 50%, low level of hairpin,dimer,loop formation, and Tm of about the same so that u can optimize the Ta more easier. If the ...

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by magicsiew
Wed Sep 29, 2010 5:09 pm
 
Forum: Molecular Biology
Topic: primer designing
Replies: 4
Views: 2850

How to get double stranded cDNA from first-strand cDNA

... seems I can use DNA Polymerase I to do it, using the hairpin loop as the primer or using the RNA leftovers as primers after RNase digestion. However, wikipedia ...

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by frankalpha
Mon Sep 20, 2010 8:08 pm
 
Forum: Molecular Biology
Topic: How to get double stranded cDNA from first-strand cDNA
Replies: 5
Views: 2541

Re: Reverse transcription of a circular RNA template

... can I use DNA Polymerase I to extend the other DNA chain, using the hairpin loop at the end of the first-strand cDNA as the primer? I'm a little confused about your 2nd ...

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by frankalpha
Mon Sep 20, 2010 5:52 pm
 
Forum: Molecular Biology
Topic: Reverse transcription of a circular RNA template
Replies: 7
Views: 3310

RNA interference

In plants for getting a hairpin loop structure in pHannibal vector people normally use cDNA as the starting material from which to amplify ...

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by tgk
Wed Aug 18, 2010 6:04 am
 
Forum: Molecular Biology
Topic: RNA interference
Replies: 3
Views: 1830
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