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Hairpin

Hairpin

The structure formed by a polynucleic acid by base-pairing between neighboring complementary sequences of a single strand of either dna or rna.


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PCR from cDNA question

... 3ul such RNA yielded no bands (not even the ribosomal RNAs). The RNA sample have been heated at 65C for 5min before electrophoresis to eliminate hairpin structures. The cDNA was obtained using iScript reverse-transcriptase, primed by random hexamers. Curiously, the same cDNA gives weird results ...

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by vastgenome
Fri Jun 22, 2012 3:55 pm
 
Forum: Molecular Biology
Topic: PCR from cDNA question
Replies: 1
Views: 3135

Reasons for Skepticism on the Endosymbiosis?

... Albert de Roos writes , [I]n linear mitochondrial chromosomes various different mechanisms to "prevent" shortening exist, ranging from hairpin loops and self-priming to protein-assisted primer synthesis (see here ). The telomeric regions of mitochondrial chromosomes do not seem to have ...

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by SuperfluousAvatar
Thu Jan 12, 2012 7:43 pm
 
Forum: Cell Biology
Topic: Reasons for Skepticism on the Endosymbiosis?
Replies: 0
Views: 6048

Renaturing/re-annealing denatured DNA strands?

By the way those circular structures, or more exactly bubbles are usually known as hairpin structures. They can be a problem when you are designing primers. They are also essential for example to the correct folding and activity of ribozymes, tRNA and of the ribosome, ...

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by canalon
Thu Jan 06, 2011 2:32 am
 
Forum: Genetics
Topic: Renaturing/re-annealing denatured DNA strands?
Replies: 5
Views: 4342

DNA Questions *PLEASE HELP*

... answer is obvious and do not need any calculations. 1b- basically the same question, just a different set of dinucleotide in the second list. 2- Hairpin is the traditional term. Think of it as self complementation. The real sequence doesn't matter, it is all about the self complementation.

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by canalon
Thu Nov 04, 2010 8:47 pm
 
Forum: Genetics
Topic: DNA Questions *PLEASE HELP*
Replies: 5
Views: 6076

DNA Oligo folding

... Do you guys know of a method that can do both at the same time? I want to know which structure that will be the most probable to occur (like hairpin or primer-template hybridization). I know I can look at the Tm and delta-G, but would also like to see the full structure of what's most probable. ...

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by Skyrre
Tue Oct 26, 2010 2:50 pm
 
Forum: Molecular Biology
Topic: DNA Oligo folding
Replies: 0
Views: 1167
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