Gum resin

... food preservative,sodium chlorite is present in toothpast,chewing gum,shampoo and aqueous cosmetic formulations,.formaldehyde is present in tobocco smoke, resins, crease resistant fabrics and aqueous cosmetic formulations such as shampoos, conditioners, ...

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Your protein is inactive due to the high concentration of imidazole. Try to use a cobalt based resin and that should do the trick. You will extract and wash in absence of imidazole and then purify in low concentration s of imidazole.

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... case you will lose all resolution and maybe even your sample if it sticks to the column and won't come off. Since the protein is bound to the resin after loading an ion exchange resin, I wouldn't think the elution would be that badly affected by a slight drying out of the top of the resin. ...

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... When using the ion exchange column in this method, why is it necessary to ensure that the liquid does not drain below the surface of the resin in the column? I cant find the answer anywhere. Does it have anything to do with disturbing the resin?.......Thanks

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... You could also try a phenol/CIAA extraction or two followed by either ethanol or isopropanol precipitation, or passing it over another tube of resin.

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