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Guidelines

Guidelines

Directions or principles presenting current or future rules of policy. Guidelines may be developed by government agencies at any level, institutions, professional societies, governing boards, or by the convening of expert panels. The text may be cursive or in outline form but is generally a comprehensive guide to problems and approaches in any field of activity. For guidelines in the field of health care and clinical medicine, practice guidelines is available.


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MolBio calculations

1 bp weights on average 650 Da (e.g. here: https://www.neb.com/tools-and-resources/usage-guidelines/nucleic-acid-data ). Thus 5.5 kbp plasmid weights 5500*650 Da. If you know the MW of the plasmid, you should know how much 1 pmol weights (in mass units) ad thus it should ...

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by JackBean
Wed Dec 04, 2013 8:12 pm
 
Forum: Molecular Biology
Topic: MolBio calculations
Replies: 1
Views: 940

Promoter PCR primer set problem

... the beginning, that seems like way too much. Both your enzymes are fine with only 1 base in the end: https://www.neb.com/tools-and-resources/usage-guidelines/cleavage-close-to-the-end-of-dna-fragments

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by JackBean
Tue Jul 09, 2013 9:29 am
 
Forum: Molecular Biology
Topic: Promoter PCR primer set problem
Replies: 2
Views: 1928

overexpression xylanase gene

1) this can have several guidelines, since bacteria have no introns, you can easily clone the gene directly from genomic DNA. This clone into any vector (either one for keeping the gene or directly into overexpression one). Transform ...

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by JackBean
Thu Dec 13, 2012 11:43 am
 
Forum: Molecular Biology
Topic: overexpression xylanase gene
Replies: 2
Views: 1247

DO NOT UNDERSTAND PRIMER DESIGN

... was not able to respond to your question earlier. Unfortunately, I just joined. Hopefully I still could provide some assistance. There are several guidelines for designing primers for PCR or sequencing. (1) The primer length should range 18 to 22 bases. Your primers are 21 bases in length and should ...

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by DougB
Wed Apr 18, 2012 2:22 pm
 
Forum: Molecular Biology
Topic: DO NOT UNDERSTAND PRIMER DESIGN
Replies: 4
Views: 2698

Designing Primer for over lapping PCR

... to design the primer for overlapping PCR. I usually used Primer Premier 6 as primer designing software. You are highly appreciated to give me some guidelines to design overlapping primer by using Primer Premier 6. Thanking you Khan

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by Rezaulbiox
Tue Feb 28, 2012 7:33 am
 
Forum: Molecular Biology
Topic: Designing Primer for over lapping PCR
Replies: 0
Views: 618
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