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Gram stain

Gram stain

(Science: microbiology, procedure) a method of staining bacteria using a violet stain. The gram staining characteristics (denoted as positive or negative) can assist in the identification of the offending bacteria.

a heat fixed bacterial smear is stained with crystal violet (methyl violet), treated with 3% iodine/potassium iodide solution, washed with alcohol and counterstained. The method differentiates bacteria into two main classes, gram-positive and gram-negative. Certain bacteria, notably mycobacteria, that have walls with high lipid content show acid-fast staining the stain resists decolouration in strong acid.


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identify bacterias in Gram stain kit &The Giemsa Stain Kit

The Gram Stain Kit is intended for the demonstration and differentiation of Gram-positive and Gram-negative bacteria. Gram ...

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by scytek
Sat Mar 15, 2014 1:11 pm
 
Forum: General Discussion
Topic: identify bacterias in Gram stain kit &The Giemsa Stain Kit
Replies: 0
Views: 1416

Re: Bacterial Taxonomy

... projections with hooked tips. I don't know how an archaean would Gram stain.

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by wildfunguy
Tue Dec 03, 2013 12:21 am
 
Forum: Microbiology
Topic: Bacterial Taxonomy
Replies: 3
Views: 4311

Bacterial Taxonomy

... make more such charts. I would particularly like a chart that relates Gram-staining to taxonomy, one that makes note of all the common exceptions. Maybe there is already ...

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by wildfunguy
Sat Oct 19, 2013 6:12 pm
 
Forum: Microbiology
Topic: Bacterial Taxonomy
Replies: 3
Views: 4311

Using oxidizing agents in gram stain

Considering the fact that Iodine is an oxidizing agent, what would happen if I replaced iodine with another oxidizing agent during the gram staining process ? So far I know that the purpose of the iodine is to form a complex with the crystal violet in order to enable the cell wall of the ...

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by ccBiologist
Sun Sep 22, 2013 2:44 pm
 
Forum: Microbiology
Topic: Using oxidizing agents in gram stain
Replies: 1
Views: 1434

gram negative cocci. please help

You should have done a gram stain ...

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by sampei
Tue May 28, 2013 2:46 pm
 
Forum: Microbiology
Topic: gram negative cocci. please help
Replies: 8
Views: 9416
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