1. (Science: botany) a fleshy, three-celled, many-seeded fruit, as the melon, pumpkin, cucumber, etc, of the order Cucurbitaceae; and especially the bottle gourd (Lagenaria vulgaris) which occurs in a great variety of forms, and, when the interior part is removed, serves for bottles, dippers, cups, and other dishes.
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... I incubate the single seed in CTAB buffer for one hour, grind with pellet pestle, and proceed as normal, no liquid nitrogen is used. With bottle gourds, I've been excising a small amount of embryo tissue and attempting the same procedure, but I'm ending up with very low 260/280 values. I added ...
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