Glycosylation

Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS? sorry, but I do not ...

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Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS?

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... envelope are more variable than those of many other viruses. Furthermore, the functionally important epitopes of the gp120 protein are masked by glycosylation, trimerisation and receptor-induced conformational changes making it difficult to block with neutralising antibodies. The ineffectiveness ...

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What problems could the need for correct post translational modification have for synthetic biology? For example, I am particularly thinking of glycosylation events and carbohydrates, which have been described as nature's third alphabet akin to nucleotides and amino acids. There are known examples ...

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... to above was probably mRNA or protein processing. Splicing is one example of this, but there are many other types of modifications as well - glycosylation and phosphorylation, for example, which occur after translation. There is no guarantee that the enzymes that do this for the protein in ...

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