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Glycosylation

Glycosylation

(Science: biochemistry) The process of adding sugar units such as in the addition of glycan chains to proteins. An occurrence where a carbohydrate is added to a protein molecule, which can occur in the golgi apparatus.


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Re:

Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS? sorry, but I do not ...

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by Bio2013
Sat Feb 16, 2013 8:48 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3273

Western Blot

Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS?

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by JackBean
Mon Feb 11, 2013 3:31 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3273

Vaccine for HIV

... envelope are more variable than those of many other viruses. Furthermore, the functionally important epitopes of the gp120 protein are masked by glycosylation, trimerisation and receptor-induced conformational changes making it difficult to block with neutralising antibodies. The ineffectiveness ...

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by suzidoc8
Mon Jul 23, 2012 2:47 pm
 
Forum: Microbiology
Topic: Vaccine for HIV
Replies: 1
Views: 7619

Problems with Synthetic Biology?

What problems could the need for correct post translational modification have for synthetic biology? For example, I am particularly thinking of glycosylation events and carbohydrates, which have been described as nature's third alphabet akin to nucleotides and amino acids. There are known examples ...

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by docholliday
Thu May 10, 2012 5:03 am
 
Forum: Molecular Biology
Topic: Problems with Synthetic Biology?
Replies: 1
Views: 1273

A question I'm finding hard to answer (recombinant protein)

... to above was probably mRNA or protein processing. Splicing is one example of this, but there are many other types of modifications as well - glycosylation and phosphorylation, for example, which occur after translation. There is no guarantee that the enzymes that do this for the protein in ...

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by AstraSequi
Mon Apr 30, 2012 5:39 pm
 
Forum: Cell Biology
Topic: A question I'm finding hard to answer (recombinant protein)
Replies: 9
Views: 6765
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