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Glycerol

Definition

noun, plural: glycerols

(1) A colorless, odorless, viscous metabolic intermediate and structural component of the major classes of biological lipids, triglycerides and phosphatidyl phospholipids.

(2) 1,2,3-trihydroxy-propane or propan-1,2,3-triol; a trihydric alcohol derived from saponification of fats and oils.


Supplement

For human consumption, glycerol is classified by the FDA among the sugar alcohols as a caloric macronutrient. It is used as antifreeze, a plasticizer, and a food sweetener and in the manufacture of dynamite, cosmetics etc.


Also called: glycerin or glycerine

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Problems making chemically competent cells

... MnCl2•4H2O (50mM) 0.998g Potassium acetate (30mM) 3ml (1M stock pH 7.5, 9.814g in 100ml solution, pH with acetic acid) CaCl2•2H2O (10mM) 0.15g Glycerol (15% w/v) 15g Final pH: 5.8 with dilute acetic acid. Don’t overshoot – if you do, start again RF2 Compound (end concentration) Amount for 100ml ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
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DNase I stability

Does anyone know exactly how long DNase I stays active, when stored in 50% glycerol and in -20C (kept in liquid form to avoid freeze/thaw cycles)? Some supplier stated that their product is guaranteed to have activity > 90% after 6 months, but others simply ...

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by biohazard
Tue Nov 06, 2012 9:30 am
 
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Topic: DNase I stability
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Re: question for evolutionary biologist.

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by scottie
Thu Sep 13, 2012 1:22 pm
 
Forum: Evolution
Topic: question for evolutionary biologist.
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Re: Question re: storing bacteria sample

Bacteria can be stored indefinitely (depending on temperature) in glycerol. Biology-online has many resources, one of which is http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=2083 . You may not have access to the equipment and ...

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by wbla3335
Sun Aug 26, 2012 4:01 am
 
Forum: General Discussion
Topic: Question re: storing bacteria sample
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Salmonela E-coli and others - for experts only

yes bacteria generally survive freezing, but it is better to preserve them in 10-20% glycerol and yes they survive in the stomach

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by triton
Wed Aug 22, 2012 7:12 pm
 
Forum: Microbiology
Topic: Salmonela E-coli and others - for experts only
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Views: 2372
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