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Glutaraldehyde

Glutaraldehyde

(Science: chemical) a dialdehyde used as a fixative, especially for electron microscopy. By its interaction with amino groups (and others) it forms cross links between proteins.


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Microorganisms and organic preservation?

... you try simply change the water for organic solvent that will evaporate? Fix the fruit with protein linking fixative like 2% paraformaldehyde or glutaraldehyde for several days. Put the fixed fruit into serie of organic solvent concentration gradient- 30% ethanol, 50% ethanol, 70% ethanol, 96% ...

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by triton
Wed Aug 22, 2012 7:01 pm
 
Forum: Microbiology
Topic: Microorganisms and organic preservation?
Replies: 1
Views: 3061

Keeping proteins from washing off the slide

... is causing the pattern to come off. I've tried washing with PBS as well as DI water. I have also tried various coatings for the slide, such as glutaraldehyde, silane derivative and agarose. The only acceptable patterning results are with bare slide. But I'm trying to keep the patterns from ...

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by TheWalkingGlitch
Fri Oct 28, 2011 8:10 am
 
Forum: Molecular Biology
Topic: Keeping proteins from washing off the slide
Replies: 5
Views: 1485

Doing research project -- trying to configure dosages...

... this year. What I plan to do is attach a Fas (death receptor ligand) to a mesothelin antibody by binding the 2 with the protein crosslinker Glutaraldehyde. This ligand-antibody combination will then be administered to pancreatic cancer cells that overexpress mesothelin. The purpose of the ...

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by HenryH
Sat Sep 12, 2009 2:59 pm
 
Forum: Molecular Biology
Topic: Doing research project -- trying to configure dosages...
Replies: 1
Views: 1092

Re: How to be "good at the bench"

... Don’t autoclave: corrosives (Acids, phenol, bases), solvents or volatiles (ethanol, methanol. Chloroform); liquids containing bleach, formalin, or glutaraldehyde; buffers with detergents, such as 10% SDS, since they can boil over; heat labile ingredients such as serum, vitamins, antibodies, and ...

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by jangajarn
Sat May 23, 2009 7:15 pm
 
Forum: General Discussion
Topic: How to be "good at the bench"
Replies: 4
Views: 18197

Re: dimer-homodimer-heterodimer

... That would be the simplest thing to do. Native gels are possible but tricky to do and interpret. Or you might try mild cross-linking with glutaraldehyde followed by SDS-PAGE. If you have purified what you believe to be active protein, you can do an N-terminal sequence determination. If ...

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by blcr11
Sat May 03, 2008 2:23 pm
 
Forum: Molecular Biology
Topic: dimer-homodimer-heterodimer
Replies: 2
Views: 2680
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