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Glutaraldehyde

Glutaraldehyde

(Science: chemical) a dialdehyde used as a fixative, especially for electron microscopy. By its interaction with amino groups (and others) it forms cross links between proteins.


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Re: How to be "good at the bench"

... Don’t autoclave: corrosives (Acids, phenol, bases), solvents or volatiles (ethanol, methanol. Chloroform); liquids containing bleach, formalin, or glutaraldehyde; buffers with detergents, such as 10% SDS, since they can boil over; heat labile ingredients such as serum, vitamins, antibodies, and ...

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by jangajarn
Sat May 23, 2009 7:15 pm
 
Forum: General Discussion
Topic: How to be "good at the bench"
Replies: 3
Views: 1441

Re: dimer-homodimer-heterodimer

... That would be the simplest thing to do. Native gels are possible but tricky to do and interpret. Or you might try mild cross-linking with glutaraldehyde followed by SDS-PAGE. If you have purified what you believe to be active protein, you can do an N-terminal sequence determination. If ...

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by blcr11
Sat May 03, 2008 2:23 pm
 
Forum: Molecular Biology
Topic: dimer-homodimer-heterodimer
Replies: 2
Views: 604

The Fiber Disease

... of yeast and mycelial fungi in culture and could thus indicate the loss of viability of MTT-negative rhinosporidial endospores. Hydrogen peroxide, glutaraldehyde, chloroxylenol, chlorhexidine, cetrimide, thimerosal, 70% ethanol, iodine in 70% ethanol, 10% formalin, povidone-iodine, sodium azide ...

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by tamtam
Mon Nov 27, 2006 3:26 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 748847

The Fiber Disease

... of yeast and mycelial fungi in culture and could thus indicate the loss of viability of MTT-negative rhinosporidial endospores. Hydrogen peroxide, glutaraldehyde, chloroxylenol, chlorhexidine, cetrimide, thimerosal, 70% ethanol, iodine in 70% ethanol, 10% formalin, povidone-iodine, sodium azide ...

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by Nadas Moksha
Mon Nov 27, 2006 4:41 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 748847

The Fiber Disease

... immobilized Agrobacterium radiobacter that produces a thermostable mannose isomerase. The cells were immobilized by adsorption on chitosan or by glutaraldehyde crosslinking in the presence of albumin. Optimum conditions for mannose isomerase activity were 60 °C and pH 7.5. Continuous reaction ...

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by London
Mon Oct 02, 2006 12:16 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 748847
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