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Glutamate

Glutamate

(Science: biochemistry, physiology) major fast excitatory neurotransmitter in the mammalian central nervous system.

See: glutamate receptor.

also the excitatory neuromuscular transmitter in arthropod skeletal muscles.


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Glutamine synthetase in animals

Hi everybody I've been looking for a while now, and haven't come across this anywhere. Is glutamine synthetase (enzyme synthesizing glutamine from glutamate and ammonia) present in heterotrophs, e.g animals? This is quite a big deal, because it is the primary pahtway of ammonia assimilation in ...

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by miikaelf
Sat Jan 12, 2013 12:33 pm
 
Forum: Molecular Biology
Topic: Glutamine synthetase in animals
Replies: 2
Views: 1243

Interpretation & Troubleshooting on SDS/Native PAGE

Dear All, I'm currently doing purification on the glutamate decarboxylase. After running both DEAE and gel filtration purification, I did both SDS and Native PAGE to verify my results. For SDS PAGE, there were 3 faint bands found and all of it are ...

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by schroddycat
Sat Dec 15, 2012 4:12 pm
 
Forum: Molecular Biology
Topic: Interpretation & Troubleshooting on SDS/Native PAGE
Replies: 1
Views: 1969

Relationship between total protein and enzyme activity

Dear all, appreciate your comments here. I'm currently extracting GAD enzyme (Glutamate decarboxylase) by doing enzymatic lysis onto the fungus Aspergillus Oryzae.The lytic enzymatic cocktail was Yatalase, which composing of Chitinase, Chitobiase, Chitosanase, ...

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by schroddycat
Sun Sep 02, 2012 12:34 pm
 
Forum: Molecular Biology
Topic: Relationship between total protein and enzyme activity
Replies: 1
Views: 4997

How much Glutamate is used to build proteins

... does it last in it's free form before it is metabolized? If you know, please share! (Trying to decipher the information available about MSG, free Glutamate, bound Glutamate, protein incorporated Glutamate, etc.,) Thanks!

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by ndtb
Thu Aug 30, 2012 9:24 pm
 
Forum: Human Biology
Topic: How much Glutamate is used to build proteins
Replies: 0
Views: 1652

Collect the cytoplasmic loop segments from 7TM receptors

... loops of '7-transmembrane helix' receptor proteins show structural homology/conserved features. I have chosen Class C (or 3) Metabotropic glutamate receptors to work on, I know roughly what genes encode different types of that receptor family proteins but I DO NOT know how to isolate sequences ...

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by studentbio
Mon Mar 19, 2012 6:00 pm
 
Forum: Bioinformatics
Topic: Collect the cytoplasmic loop segments from 7TM receptors
Replies: 1
Views: 2523
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