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Dictionary » G » Glutamate GlutamateGlutamate (Science: biochemistry, physiology) major fast excitatory neurotransmitter in the mammalian central nervous system. See: glutamate receptor. also the excitatory neuromuscular transmitter in arthropod skeletal muscles. ![]()
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Results from our forumGlutamine synthetase in animalsHi everybody I've been looking for a while now, and haven't come across this anywhere. Is glutamine synthetase (enzyme synthesizing glutamine from glutamate and ammonia) present in heterotrophs, e.g animals? This is quite a big deal, because it is the primary pahtway of ammonia assimilation in ...
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Interpretation & Troubleshooting on SDS/Native PAGEDear All, I'm currently doing purification on the glutamate decarboxylase. After running both DEAE and gel filtration purification, I did both SDS and Native PAGE to verify my results. For SDS PAGE, there were 3 faint bands found and all of it are ...
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Relationship between total protein and enzyme activityDear all, appreciate your comments here. I'm currently extracting GAD enzyme (Glutamate decarboxylase) by doing enzymatic lysis onto the fungus Aspergillus Oryzae.The lytic enzymatic cocktail was Yatalase, which composing of Chitinase, Chitobiase, Chitosanase, ...
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How much Glutamate is used to build proteins... does it last in it's free form before it is metabolized? If you know, please share! (Trying to decipher the information available about MSG, free Glutamate, bound Glutamate, protein incorporated Glutamate, etc.,) Thanks!
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Collect the cytoplasmic loop segments from 7TM receptors... loops of '7-transmembrane helix' receptor proteins show structural homology/conserved features. I have chosen Class C (or 3) Metabotropic glutamate receptors to work on, I know roughly what genes encode different types of that receptor family proteins but I DO NOT know how to isolate sequences ...
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