Giemsa

I need references to stain spermatozoa in order to investigate their viability/vitality. I read that double-staining (trypan blue/congo red + Giemsa) is good to differ dead/alive and also the presence of acrosome. Triple-staining (eosin/nigrosin+Bismarck brown+...(I forgot the third dye)) is ...

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... anaerobic conditions7,8. C. piliforme stains weakly with hematoxylin and eosin, so confirmation is frequently made using silver impregnation or Giemsa stains. The vegetative form, readily visible microscopically, is large and ranges from 8-40 mm in length. The organism does not grow in cell-free ...

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... difficulty identifying the cells of our experimental cultures. Our control was muscle tissue if that helps at all and the attached photos are giemsa stained cultures under an inverted microscope (20x). Can anyone help me identify these? Are they just mutants? I've been looking at different ...

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