Dictionary » G » Genomic dna

Genomic dna

Genomic dna

(Science: molecular biology) The dna which is found in the organisms genome and is passed on to offspring as information necessary for survival. The phrase is used to distinguish between other types of dna, such as found within plasmids.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


How

... in those (easy) then excise the double insert and transfer it to the genomic DNA as you would do. If you want to be more clever you can use a super PCR (I highly recommend ...

See entire post
by canalon
Sat Nov 02, 2013 6:15 pm
 
Forum: Molecular Biology
Topic: How
Replies: 4
Views: 903

Re: Does complementary DNA include only coding sequences ?

Right - another technique, like RACE, is usually needed to extend the sequence across UTRs. If the primers are designed against genomic sequence, they can include UTR sequence, but are unlikely to capture the entire UTRs.

See entire post
by jonmoulton
Tue Oct 29, 2013 3:14 pm
 
Forum: Genetics
Topic: Does complementary DNA include only coding sequences ?
Replies: 4
Views: 1044

Aligning RNA to genomic DNA

... to do is to align an RNA seqeunce which is compose of exon 1 and 2 to a genomic DNA - however so far it I can only align either exon 1 or 2 but not both because there is a ...

See entire post
by simplitia
Wed Sep 25, 2013 6:53 pm
 
Forum: Bioinformatics
Topic: Aligning RNA to genomic DNA
Replies: 1
Views: 730

BL21 colony as a template

... I want to amplify a 2kb product from BL21 cells. I do not have genomic DNA of BL21 so, I want to do colony PCR where colony as a template. Can anyone suggest me whether ...

See entire post
by sureshG
Mon Jul 15, 2013 10:14 am
 
Forum: Molecular Biology
Topic: BL21 colony as a template
Replies: 0
Views: 383

Promoter PCR primer set problem

... gggg TCTAGA CAA TGT ACA CCA ACA TAT AC -3' (Xbai) Tm 56.5 I am using genomic DNA as template and KOD kit. Unfortunately I have been unsuccessful in amplifying the target ...

See entire post
by bravebeaker
Tue Jul 09, 2013 3:05 am
 
Forum: Molecular Biology
Topic: Promoter PCR primer set problem
Replies: 2
Views: 1897
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 7,547 times. 
What links here | Related changes | Permanent link