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Gene product

Gene product

The biochemical material, either rna or protein, resulting from expression of a gene. The amount of gene product is used to measure how active a gene is, abnormal amounts can be correlated with disease-causing alleles.


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Gateway technology

... and expression vectors based on the property of phages to recombine its genetic material with bacterias. However, why do I need to perform the BP ... an expression clone of a certain gene? Why can't I use an attL-PCR product or a linearized attL DNA and make a reaction with the destination ...

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by miibay
Tue Aug 19, 2014 10:47 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 83

Help: Confused with primers in a PCR of a vector?

... I'm not entirely familiar with what is going on. We recently ligated a gene into a pBS vector and popped it into some E. coli cells. We looked for ... off is just where these primers will bind to, and what the subsequent product will be. The one that really throws me off is number 2, because it ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2219

Do chromosomses ever get competitive?

... there could be a pathway between the dominant and recessive protein products. I will speculate about how such a pathway might arise. Suppose ... not genotypes, the heterozygous genotype persists. Then, in comes a new gene from somewhere else in the genome. It produces protein product "C". ...

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by wildfunguy
Sun Sep 29, 2013 2:50 pm
 
Forum: Cell Biology
Topic: Do chromosomses ever get competitive?
Replies: 9
Views: 6657

Gene deletion

... for some amino acid or vitamine or something. And you use the missing gene instead of antibiotic resistance gene, so that your transformants are ... one). However, if they added 5-fluoro-uracil, it produced some toxic product and thus only fungi lacking the selection gene survived.

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by JackBean
Thu Sep 05, 2013 3:31 pm
 
Forum: Molecular Biology
Topic: Gene deletion
Replies: 5
Views: 2186

Re:

... I think one of the reasons for dominance in traits is that d dominant gene tends to inhibit the expression of the recessive gene either directly ... the recessive allele is still coding for an under-performing protein product. But since the protein's job is to serve as an inhibitor (perhaps ...

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by wildfunguy
Thu Aug 22, 2013 6:33 pm
 
Forum: Cell Biology
Topic: Do chromosomses ever get competitive?
Replies: 9
Views: 6657
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