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Gene amplification

Definition

noun

The process of duplicating a particular gene, causing the gene to increase in frequency inside the cell, as well as amplifying the phenotype (e.g. the proteins) that the gene confers (or code for).


Supplement

Gene amplification is one way by which a gene can be over-expressed. It is also linked to drug resistance in cancer cells where amplification results in cells being prevented from absorbing the chemotherapeutic drug.

Gene amplification may also occur artificially, as in by polymerase chain reaction technique.


Synonym: gene duplication.


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amplification in no RT control

I am doing some qRT PCR to assess changes in bacterial gene expression following exposure to antimicrobials. RNA has been isolated by ... and finally been DNase treated prior to reverse transcription. I am getting amplification in all of my no RT controls (4 treatments and an untreated), although ...

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by biomart
Fri Mar 20, 2015 1:58 am
 
Forum: General Discussion
Topic: amplification in no RT control
Replies: 0
Views: 39

Re: Any ideas for vaccine development or anti-viral drugs???

... Iversen PL, Bavari S, Muhlberger E. VP35 Knockdown Inhibits Ebola Virus Amplification and Protects against Lethal Infection in Mice. Antimicrob Agents ... DK, Pratt WD, Blouch R, Stein DA, Aman MJ, Iversen PL, Bavari S. Gene-Specific Countermeasures against Ebola Virus Based on Antisense Phosphorodiamidate ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 12572

Re: An interesting PCR smearing...I dont know what to do :(

... I am using 3 different primers to amplify 3 different exons of a gene . 1 of the exon is amplifying very well ( a single bright band ) but the other two exon amplification is not coming properly.and this has occured recently. ( even though the ...

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by priyah
Sat Jun 28, 2014 5:20 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 8779

Pyrosequencing

Hello, I would like to sequence a gene region with a couple of mutation hot spots nearby each other, and I have a few questions. I guess after PCR amplification of the gene region of interest, I should use a sequencing primer that anneals to ...

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by kk
Wed Jan 23, 2013 3:08 pm
 
Forum: Molecular Biology
Topic: Pyrosequencing
Replies: 2
Views: 2930

Re: Unidirectional ORI, Onion-Skin-Model

... below. The paper also says that the ORI lies in the middle of the gene. The illustration looks like the replication went both ways. From my ... two directions. So it has to lie either at the right or the left of the amplification bubbles... "Unlike the amphibian rRNA genes in Example ...

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by Rost
Thu Aug 30, 2012 6:52 am
 
Forum: Genetics
Topic: Unidirectional ORI, Onion-Skin-Model
Replies: 4
Views: 8211
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