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Dictionary » G » Gene amplification

Gene amplification

Definition

noun

The process of duplicating a particular gene, causing the gene to increase in frequency inside the cell, as well as amplifying the phenotype (e.g. the proteins) that the gene confers (or code for).


Supplement

Gene amplification is one way by which a gene can be over-expressed. It is also linked to drug resistance in cancer cells where amplification results in cells being prevented from absorbing the chemotherapeutic drug.

Gene amplification may also occur artificially, as in by polymerase chain reaction technique.


Synonym: gene duplication.


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Mutation

... might be multiple answers for a blank). 1). Point mutation on the gene ..................... 2). Chromosomal rearrangement that involved the gene .................... 3). Gene amplification ..................... 4). Oncogenic retrovirus insertion ..................... ...

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by whizzbee
Sun Mar 01, 2009 3:42 pm
 
Forum: Molecular Biology
Topic: Mutation
Replies: 0
Views: 451

Re: PCR for long DNA fragment

For amplification of long templates, use specific long PCR amplification polymerases with proof ... activity, like Expand Hi Fi Polymerase (Roche). If you have trouble in amplifying the gene as a 6 kb single fragment , try designing primers to amplify gene as fragments ( eg two ...

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by aviprem
Mon Aug 04, 2008 1:16 pm
 
Forum: Molecular Biology
Topic: PCR for long DNA fragment
Replies: 4
Views: 1806

PCR

hi everybody In order to study the role of some genes, I have designed gene specific primers and made a PCR. And I separated the ... which was done without using cDNA template(which was for the confirmation of amplification). Can anybody answer me, how will get band even without template.

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by shivakumar
Wed May 14, 2008 11:18 am
 
Forum: Molecular Biology
Topic: PCR
Replies: 1
Views: 527

problem with cloning using pTYB2

I am trying to clone a 300 bp gene into pTYB2 vector (7.4kb)for later protein purification method by IMPACT-CN. NdeI ... corresponding to the N- and C- termini of the gene and incorporated by PCR amplification. The PCR product and the vector were both double digested with SmaI and ...

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by ripplemm
Mon May 05, 2008 8:57 pm
 
Forum: Molecular Biology
Topic: problem with cloning using pTYB2
Replies: 3
Views: 1304

gene cloning and amplification

Sorry, the other half of the question: You don't need to amplify the gene sequence after you've cloned it in order to express it. You clone the ... You may induce expression especially when using E coli, but that's not amplification of the sequence. Induction is turning on gene expression by ...

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by blcr11
Wed Apr 23, 2008 2:01 pm
 
Forum: Molecular Biology
Topic: gene cloning and amplification
Replies: 3
Views: 487
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