Dictionary » G » Gene amplification

Gene amplification

Definition

noun

The process of duplicating a particular gene, causing the gene to increase in frequency inside the cell, as well as amplifying the phenotype (e.g. the proteins) that the gene confers (or code for).


Supplement

Gene amplification is one way by which a gene can be over-expressed. It is also linked to drug resistance in cancer cells where amplification results in cells being prevented from absorbing the chemotherapeutic drug.

Gene amplification may also occur artificially, as in by polymerase chain reaction technique.


Synonym: gene duplication.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Pyrosequencing

Hello, I would like to sequence a gene region with a couple of mutation hot spots nearby each other, and I have a few questions. I guess after PCR amplification of the gene region of interest, I should use a sequencing primer that anneals to ...

See entire post
by kk
Wed Jan 23, 2013 3:08 pm
 
Forum: Molecular Biology
Topic: Pyrosequencing
Replies: 2
Views: 1755

Re: Unidirectional ORI, Onion-Skin-Model

... below. The paper also says that the ORI lies in the middle of the gene. The illustration looks like the replication went both ways. From my ... two directions. So it has to lie either at the right or the left of the amplification bubbles... "Unlike the amphibian rRNA genes in Example ...

See entire post
by Rost
Thu Aug 30, 2012 6:52 am
 
Forum: Genetics
Topic: Unidirectional ORI, Onion-Skin-Model
Replies: 4
Views: 5352

Unidirectional ORI, Onion-Skin-Model

... . I understand why D. melanogaster amplificates its chorion genes etc. I always read that the onion skin model uses an unidirectional ... which i've looked at is always placed in the center of the amplification bubbles. The amplification (so as the replication?) however ...

See entire post
by Rost
Wed Aug 29, 2012 1:38 pm
 
Forum: Genetics
Topic: Unidirectional ORI, Onion-Skin-Model
Replies: 4
Views: 5352

Re: restriction analysis

hello I am about to start and my first step would be the amplification of my desired gene.. and then digestiona nd ligation into expression vector.. so If i start from the first step... ...

See entire post
by siddharthsameer
Tue Jul 17, 2012 1:31 pm
 
Forum: Molecular Biology
Topic: restriction analysis
Replies: 14
Views: 5894

Colony PCR

... the usual speed is ~1000 bases per minute, I would try 4 minute long amplification. Another possibility is to use other primers, maybe e.g. M13 forward + some reverse primer which binds in the beginning of your gene.

See entire post
by JackBean
Fri Dec 02, 2011 7:39 am
 
Forum: Molecular Biology
Topic: Colony PCR
Replies: 4
Views: 3252
View all matching forum results

This page was last modified 09:11, 10 December 2008. This page has been accessed 3,212 times. 
What links here | Related changes | Permanent link