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Gene Cloning

The man-operated process of extracting dna from a host cell and implanting it to function in another type of cell.


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Re: Gene cloning question

I want to clone a human gene in pET-21a, and transfer to E.coli. I clone the gene in Nde 1 site that it’s sequence is CA/TATG, My question, is The ATG of Nde1 act as a start codon in result I must delete The ATG of my sequence or I can ...

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by mohajeri
Thu Feb 06, 2014 10:01 pm
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 20
Views: 15558

Gene Cloning

Since a PCR product can insert in both direction in the PTZ57 vector and both of clones can grow in a ampicillin LB plates, How can we find the clone that has the correct direction for expression of PCR product?

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by ghaemi100
Fri Jun 21, 2013 9:39 am
 
Forum: Molecular Biology
Topic: Gene Cloning
Replies: 3
Views: 1024

Gene patents may hamper innovations in patient care

Gene patents are discoveries, not inventions and patenting discoveries may hamper future innovations ... patient care products and services. Scientific and technological innovations in molecular cloning, sequencing, PCR, bioinformatics, biochemical methods etc., have created innovative ...

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by TMJohnson
Mon Dec 17, 2012 11:38 am
 
Forum: General Discussion
Topic: Gene patents may hamper innovations in patient care
Replies: 0
Views: 877

Re:

... 4 sets of primers. I was going to amplify 4 overlapping regions of the gene, run them on a gel, cut out the bands of expected size, purify them, ... and look at the splicing nature of ligase 6 before we re-attempt any cloning or over-expression. In order to investigate the splicing nature of ...

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by polkadotshorts
Mon Dec 10, 2012 7:12 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 2507

Re:

... germination rates under high stress conditions etc. I have suggested cloning it (with introns in) to my supervisor but he said that getting the ... some content about the alternative splicing nature of the ligase 6 gene from a gel image that I have taken. If you have the time, I would really ...

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by polkadotshorts
Sat Dec 08, 2012 3:52 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 2507
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