Gels

... different nutrient agars. I thought you could use one agar (the yellowish one) for all cultures, but I've seen sets with different colored agar gels. What are the different types used for? See here . How do I heat the agar liquid if I'm buying it in bottles, not prepared in the dish? Is there ...

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Sadly I did not help us out. In our lab often we want to check early if we have good product/restriction pattern. And with GelRed gels have to run longer to get nice bands. But it helps indeed if you run longer. Then result can be nice.

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Just saw the gels. Type of marker makes a difference. If bands are close, the gel is easily overloaded. Overall, 0.5x buffer does not help. GelRed is out of here I think. Markers keep looking bad ...

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According to your gels, which are bit poorly described in the post, Bob is AS ans Sally AA. If both genes, M and D are located in the same chromosome, chances are ~25/50% that a kid gets AS and 0% to get anemia, unless one gene mutation ...

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Ive got one gel with molecular weight marker: Phosphorylase B, BSA, Ovalbumin, Carbonic anhydrase, Lactoglobulin and Aprotinin. Then ive got 4 gels with same protein of 150 Kda giving two bands of 50 KDa and 25 KDa. is there anyway i can draw graphs from this data?

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