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Gels

Gels

Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquifies; the resulting colloid is called a sol.


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separation problem with GelRed

... to your questions. GelRed is designed to be a large dye to increase its safety. Therefore, its large size may affect DNA migration in prestain gels, leading to some separation problems. Here are some solutions to improve band separation: 1. Reduce the amount of loaded DNA. Overloading of DNA ...

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by Biotium
Wed Nov 26, 2014 4:43 pm
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 19576

Re: Western transfer failure

... transfer buffer ingredients too, I had this transfer problem once when I forgot to add SDS in transfer buffer. I was transferring two 12% SDS-PAGE gels to PVDF in one tank. 100V, 1 hr. One gel transferred fine, another went backwards to blot paper after 1 hr. Did I just arrange backwards? Did lack ...

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by yanggers
Thu Feb 06, 2014 9:16 pm
 
Forum: Molecular Biology
Topic: Western transfer failure
Replies: 5
Views: 6402

Asking for help with electrophoresis

... the samples on the side of the gel look like being “drifted down”. If I ran same samples for all wells, they would show a “sad face” pattern. The gels were ran with: 1.2% ~1.4% agarose gel, 0.5X TBE buffer (I made the buffer twice, similar results), 4~5 V/cm, and the room temperature was absolutely ...

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by ll2013
Thu Jun 06, 2013 1:09 am
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 3342

Basics for preparing bacterial cultures

... different nutrient agars. I thought you could use one agar (the yellowish one) for all cultures, but I've seen sets with different colored agar gels. What are the different types used for? See here . How do I heat the agar liquid if I'm buying it in bottles, not prepared in the dish? Is there ...

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by animus
Sun Nov 25, 2012 10:11 pm
 
Forum: Microbiology
Topic: Basics for preparing bacterial cultures
Replies: 4
Views: 3896

Re: separation problem with GelRed

Sadly I did not help us out. In our lab often we want to check early if we have good product/restriction pattern. And with GelRed gels have to run longer to get nice bands. But it helps indeed if you run longer. Then result can be nice.

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by citroenboom
Wed Nov 14, 2012 1:41 pm
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 19576
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