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Gel transfer

Gel transfer

Any lab technique used to transfer substances which had been separated using gel electrophoresis from the gel to a membrane for further processing or analysis. For example: any type of blotting.


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Re: Western transfer failure

Check the transfer buffer ingredients too, I had this transfer problem once when I forgot to add SDS in transfer buffer. I was transferring two 12% SDS-PAGE gels to PVDF in one tank. 100V, 1 hr. One gel transferred fine, another went backwards to blot paper ...

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by yanggers
Thu Feb 06, 2014 9:16 pm
 
Forum: Molecular Biology
Topic: Western transfer failure
Replies: 5
Views: 5010

Radio-phosphate binding assay

... through a native PAGE (I have combs this big). 3) Western blotting: transfer to an activated nitrocellulose/PVDF membrane). 4) Incubate blot ... control, I passed a dye (10ul), that binds phosphate, through the gel and blot. I see binding in this case. I have attached the two phosphor ...

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by vader1941
Tue Jul 10, 2012 3:32 pm
 
Forum: Molecular Biology
Topic: Radio-phosphate binding assay
Replies: 0
Views: 1530

Re: Western Blot TroubleShooting

... heat at 100C in a sand bath for 10 min. I run my samples on a SDS-PAGE gel (7.5%) for 45- 1 hour at 150 V. I use the SDS-PAGE ladder as a guideline for how long I run the gel. I transfer onto a nitrocellulose membrane. I use a solution that is transblot solution, ...

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by mutant11
Mon Nov 21, 2011 3:11 am
 
Forum: Molecular Biology
Topic: Western Blot TroubleShooting
Replies: 7
Views: 3198

Western Blot TroubleShooting

... antibody are you using, how are you separating (native vs. SDS-page gel), what kind of transfer are you doing, etc etc.

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by daniel.kurz
Mon Nov 21, 2011 2:35 am
 
Forum: Molecular Biology
Topic: Western Blot TroubleShooting
Replies: 7
Views: 3198

Re:

As far as I know the use of any kind of antiseptic hand gels is not advised in normal daily life. Your hands already contain a huge amount ... poisoning bacteria (like Staphylococcus aureus ) and with bad luck you can transfer them into food you prepare, increasing the chances that it becomes spoiled. ...

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by blackmantis
Thu Sep 23, 2010 12:52 am
 
Forum: Human Biology
Topic: Alcohol versus food poisoning bacteria
Replies: 12
Views: 25314
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