Gel transfer

... through a native PAGE (I have combs this big). 3) Western blotting: transfer to an activated nitrocellulose/PVDF membrane). 4) Incubate blot ... control, I passed a dye (10ul), that binds phosphate, through the gel and blot. I see binding in this case. I have attached the two phosphor ...

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... heat at 100C in a sand bath for 10 min. I run my samples on a SDS-PAGE gel (7.5%) for 45- 1 hour at 150 V. I use the SDS-PAGE ladder as a guideline for how long I run the gel. I transfer onto a nitrocellulose membrane. I use a solution that is transblot solution, ...

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... antibody are you using, how are you separating (native vs. SDS-page gel), what kind of transfer are you doing, etc etc.

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As far as I know the use of any kind of antiseptic hand gels is not advised in normal daily life. Your hands already contain a huge amount ... poisoning bacteria (like Staphylococcus aureus ) and with bad luck you can transfer them into food you prepare, increasing the chances that it becomes spoiled. ...

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As far as I know the use of any kind of antiseptic hand gels is not advised in normal daily life. Your hands already contain a huge amount ... poisoning bacteria (like Staphylococcus aureus ) and with bad luck you can transfer them into food you prepare, increasing the chances that it becomes spoiled. ...

See entire post