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Gel

Gel

jelly like material formed by the coagulation of a colloidal liquid. Many gels have a fibrous matrix and fluid filled interstices: gels are viscoelastic rather than simply viscous and can resist some mechanical stress without deformation.

Examples are the gels formed by large molecules such as collagen (and gelatin), agarose, acrylamide and starch.


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Re: Gel electrophoresis

haha, so there is a high concentration of the small fragments?

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by downingrock
Thu Mar 13, 2014 1:08 am
 
Forum: Genetics
Topic: Gel electrophoresis
Replies: 3
Views: 236

Gel electrophoresis

Hey guys! Would anyone know, in each of the Lane 2's, why there is a very large bright fragment that doesn't really confine to the shape of a rectangle? Just a general description would be helpful. Thank you!

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by downingrock
Wed Mar 12, 2014 12:44 pm
 
Forum: Genetics
Topic: Gel electrophoresis
Replies: 3
Views: 236

Re: Western transfer failure

... transfer buffer ingredients too, I had this transfer problem once when I forgot to add SDS in transfer buffer. I was transferring two 12% SDS-PAGE gels to PVDF in one tank. 100V, 1 hr. One gel transferred fine, another went backwards to blot paper after 1 hr. Did I just arrange backwards? Did lack ...

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by yanggers
Thu Feb 06, 2014 9:16 pm
 
Forum: Molecular Biology
Topic: Western transfer failure
Replies: 5
Views: 3993

plasmid extraction on 1.5% agarose gel

So I have two bands, the smaller that is around 8-9kb based on the marker ( my plasmids should be 8-9 kb), what is the upper band? Also I have three plasmids and I did this in a set of four replicates

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by cah2010
Wed Dec 18, 2013 2:23 am
 
Forum: Molecular Biology
Topic: plasmid extraction on 1.5% agarose gel
Replies: 5
Views: 815

electrophoretic mobility shift assay (EMSA)

... cell? Could you try it somewhere else? What do you mean by that you cannot see the blue front? It cannot just disappear. Did you try to stain your gel with silver/Coomassie?

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by JackBean
Mon Nov 11, 2013 1:50 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 645
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