Gel

You should measure from the very top of the gel. Did you plot it against log of MW?

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Also, here are my Rf values (in the x column): 0.0068 250000 0.021 150000 0.048 100000 0.075 75000 0.15 50000 0.24 37000 0.4 25000 0.49 20000 0.67 15000 0.9 10000

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Hello, I have to calculate the Rf for the molecular weight markers in lane one of the gel that I attached. When I do this and graph the Rf values against the molecular weight my graph is not linear. I marked in red where I am measuring from. Can someone please tell ...

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... weight of proteins when running a SDS-PAGE. I have conjugated several different molecular weight proteins with dyes and ran them on a 12% gel. There is no problem with the size when I loaded each protein into a separate well, but when I combined all of the proteins together into 1 well, ...

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... doesn't cut between every histone; then you would see some double-length fragments, triple-length fragments etc. If you run the fragments out on a gel you would see a ladder and analysis of the masses of the ladder rungs would reveal that they are integer multiples of the mass of the smallest rung ...

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