Gel

Well I'm no expert on this, but I would have thought the two key steps are the physical ones, the gel and the centrifugation. Have you tried running the gel at a lower voltage over a longer period, and also centrifuging for longer. Also what is the source of the DNA, can ...

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My protocol of the PAGE gel running and DNA recovery is as follows, 1. 20% native PAGE gel (The DNA i want to purify is around 20bp dsDNA + 10 bp ssDNA ) 2. running at 250 v for 30mins, 3. stain and cut the desired band; 4. cursh the ...

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... the protonated forms or the unprotonated forms. but for some reason, people don't seem to distinguish. i can't say really, i only use EDTA for DNA gel buffer, and that stuff is ready made.

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... d) Alcaligenes xylosoxidans My bacteria test results are these on API 20 E: 0204042 ONPG - ADH - LDC - ODC - CIT + H2S - URE - TDA - IND - VP - GEL - GLU + MAN - INO - SOR - RHA - SAC - MEL + AMY - ARA + Please reply as soon as possible time is running out lol every tip is appreciated.

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... that was targeting to see if there was mtDNA in the Eggs... our lab is out of Agarose right now so i cant run it and some other samples out on a gel to see if it worked. However if it didn't work in any way im going to try it agian with out the SDS in case that messed with the reaction, and if ...

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