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Gel

Gel

jelly like material formed by the coagulation of a colloidal liquid. Many gels have a fibrous matrix and fluid filled interstices: gels are viscoelastic rather than simply viscous and can resist some mechanical stress without deformation.

Examples are the gels formed by large molecules such as collagen (and gelatin), agarose, acrylamide and starch.


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Some problems of DNA recovery from native PAGE gel

Well I'm no expert on this, but I would have thought the two key steps are the physical ones, the gel and the centrifugation. Have you tried running the gel at a lower voltage over a longer period, and also centrifuging for longer. Also what is the source of the DNA, can ...

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by domwood
Wed May 20, 2009 10:25 am
 
Forum: Genetics
Topic: Some problems of DNA recovery from native PAGE gel
Replies: 1
Views: 113

Some problems of DNA recovery from native PAGE gel

My protocol of the PAGE gel running and DNA recovery is as follows, 1. 20% native PAGE gel (The DNA i want to purify is around 20bp dsDNA + 10 bp ssDNA ) 2. running at 250 v for 30mins, 3. stain and cut the desired band; 4. cursh the ...

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by wusilinqi
Thu May 14, 2009 5:20 am
 
Forum: Genetics
Topic: Some problems of DNA recovery from native PAGE gel
Replies: 1
Views: 113

Prep. of 50X TAE buffer

... the protonated forms or the unprotonated forms. but for some reason, people don't seem to distinguish. i can't say really, i only use EDTA for DNA gel buffer, and that stuff is ready made.

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by MrMistery
Thu May 07, 2009 4:47 pm
 
Forum: Molecular Biology
Topic: Prep. of 50X TAE buffer
Replies: 7
Views: 367

Trouble Identifying an Unkown Bacteria.

... d) Alcaligenes xylosoxidans My bacteria test results are these on API 20 E: 0204042 ONPG - ADH - LDC - ODC - CIT + H2S - URE - TDA - IND - VP - GEL - GLU + MAN - INO - SOR - RHA - SAC - MEL + AMY - ARA + Please reply as soon as possible time is running out lol every tip is appreciated.

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by Grillote2
Wed May 06, 2009 11:35 pm
 
Forum: Microbiology
Topic: Trouble Identifying an Unkown Bacteria.
Replies: 1
Views: 84

Can you use mtDNA to...

... that was targeting to see if there was mtDNA in the Eggs... our lab is out of Agarose right now so i cant run it and some other samples out on a gel to see if it worked. However if it didn't work in any way im going to try it agian with out the SDS in case that messed with the reaction, and if ...

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by Swordy
Wed Apr 29, 2009 5:33 pm
 
Forum: Genetics
Topic: Can you use mtDNA to...
Replies: 12
Views: 481
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