Dictionary » G » Gas-liquid chromatography

Gas-liquid chromatography

Definition

noun

A form of column chromatography where the mobile phase is a gas whereas the stationary phase is a liquid, e.g. a liquid silicone material as small beads packed in a long column


Supplement

This technique is usually applied in industrial settings and research in chemistry. It is not a common technique in biochemical processes because high molecular weight biomolecules, such as amino acids are easily denatured from heat during the process.


Abbreviation: GLC

Compare:


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Dating of blood stains

... please please let us know!): ⋅ Try to seperate the HbO2 molecule from the met-HB molecule (the difference is just Fe2+/Fe3+) using ion chromatography (or some kind of chromatography) and detect them. (Maybe an other option would be a DAD, to detect at different wavelenghts.. however ...

See entire post
by wptmdoorn
Tue Jan 28, 2014 12:32 pm
 
Forum: Human Biology
Topic: Dating of blood stains
Replies: 1
Views: 2007

Interpretation & Troubleshooting on SDS/Native PAGE

... didn't really enter the gel. What percentage was the gel? The bands on SDS-PAGE are pretty faint. How does your sample look now on gel exclusion chromatography? What was source of your protein? I guess it wasn't native enzyme, because then you could hardly have only three bands (unless you've ...

See entire post
by JackBean
Sun Dec 16, 2012 10:17 pm
 
Forum: Molecular Biology
Topic: Interpretation & Troubleshooting on SDS/Native PAGE
Replies: 1
Views: 1969

Scientific paper on DNA replication & gel electrophoresis

... 6 minutes. Strands that migrated to the top of the gel indicate a stalled replication fork. This was the bulk reaction. -Then used gel-filtration chromatography to remove all unbound proteins and then readded only the 3 proteins that were known to be necessary during replication. The same procedure ...

See entire post
by dekk1525
Sat Sep 08, 2012 11:17 pm
 
Forum: Molecular Biology
Topic: Scientific paper on DNA replication & gel electrophoresis
Replies: 0
Views: 4923

Why dialyse DMEM before protein purification (Ni-Affinity)?

... etc. but somehow I don't get why it is necessary here - what is in DMEM that would make it difficult (impossible?) to run the ni-affinity chromatography? Would the protein precipitate? If yes, why then? I've been using google for the past days with different combinations of keywords and ...

See entire post
by LittleBird
Sun May 20, 2012 3:43 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 2782

empty 1-2ml chromatography columns

Hi guys, I am looking for 1-2 ml empty chromatography columns into which I could load my Sephadex beads... Do you have any idea where can I get them? GE for example only sells 8 ml columns which are a bit too big for my needs... Arik

See entire post
by pushel
Sun Mar 04, 2012 2:53 pm
 
Forum: General Discussion
Topic: empty 1-2ml chromatography columns
Replies: 3
Views: 1196
View all matching forum results

This page was last modified 15:34, 16 February 2011. This page has been accessed 519 times. 
What links here | Related changes | Permanent link