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Galactosidase

Galactosidase

(Science: enzyme) One of a class of enzymes which cuts the glycosidic bond between the sugar galactose and another sugar molecule (of a different type than galactose).

there are two subclasses - the alpha galactosidases and beta galactosidases - which cuts the two types of glycosidic bonds (alpha and beta, respectively). An enzyme responsible for the breaking down of galactose.


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Enzymes and ATP

... having troubles with a couple questions. Lacatse hydrolyzes lactose into glucose and galactose. Galactose is then converted to another glucose by galactosidase. Outline the reaction involving these enzymes using a word equation. I initially thought: LactASE + H2o -> glucose and fructose and ...

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by twenty10
Sun Dec 14, 2008 11:03 pm
 
Forum: Molecular Biology
Topic: Enzymes and ATP
Replies: 3
Views: 781

SDS-PAGE and specific activity

... say that there is a slight increase... I've already searched on google but all I found was this sentence: "In analyzing our purification of ß-galactosidase, we will compare the number of protein bands present on an SDS PAGE gel, stained with Coomassie Blue, for each of the different stages ...

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by faerstd
Sun Nov 30, 2008 3:57 pm
 
Forum: General Discussion
Topic: SDS-PAGE and specific activity
Replies: 1
Views: 462

My undergraduate thesis project - Lactose metabolism

... towards this bacteria until the genus, because at least I have to know which phylum that this bacteria belong to. After that I want to do an beta-galactosidase enzyme assay for this bacteria and compare it with the same assay on E.coli strain K12. Any suggestion or addition about this? anything ...

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by victor
Wed Nov 05, 2008 2:09 pm
 
Forum: Microbiology
Topic: My undergraduate thesis project - Lactose metabolism
Replies: 2
Views: 911

My undergraduate thesis project - Lactose metabolism

... was still red (no pH change) and the bacteria grew there. Then I think that lactose in bacteria is cleaved into glucose and galactose by beta-galactosidase, where galactose is converted into glucose either via Leloir pathway like in E.coli or tagatose pathway like in lactobacilli. So, if the ...

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by victor
Tue Nov 04, 2008 3:10 pm
 
Forum: Microbiology
Topic: My undergraduate thesis project - Lactose metabolism
Replies: 2
Views: 911

Re: Difference between ICC and IF

... emitting photons or producing a fluorochrome. A few examples are using secondary antibodies conjugated with horseradish peroxidase or with beta-galactosidase, each of which can catalyze reactions producing stains and the stains detected by microscopy. Lumogenic substrates can emit photons when ...

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by jonmoulton
Tue Sep 16, 2008 3:37 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 2
Views: 751
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