Fusion

I'd say it's in opposite direction to abolish transcriptional fusion.

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... gene under a different promoter? If not, what is the use of placing a selectable marker and a reporter gene under the same construct (without a fusion) ? Thank you Regards :)

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Hi All, I am having the following problem. I work with a fusion gene and need to do a QPCR. I found a couple of papers with primers I can use but have no idea how people manage to design those primers and what is the product length etc.

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... unknown). He has a strain with a deletion of the chromosomal cbbA gene and containing a low copy number plasmid with a cbbA:lacZ transcriptional fusion (the strain therefore lacks a functional cbbA gene). While -galactosidase activity in this strain is low, spontaneous mutants appear with high ...

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try this http://biology.about.com/od/cellularprocesses/ss/diffusion.htm http://biology.about.com/od/cellularprocesses/ss/diffusion_2.htm if you'll still have troubles understanding, tell us what's your problem.

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