Freezing

Just put them on ice. I saw recently recomendation to keep them on freezing block (-20°C) to keep them alive longer, but I haven't tried it.

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yes bacteria generally survive freezing, but it is better to preserve them in 10-20% glycerol and yes they survive in the stomach

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... that mRNA is stable for your genes of interest. Possible solution -- increase number of cycles. 2. Primers. If you used primers many times before, freezing-thawing can affect their quality. Making new primers from stocks can help. I assume you checked specificity but might be worth looking again. ...

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... colonies? I mean do I need to re-plate them or can I just use a small amount of the liquid that I saved to grow overnight in LB broth? Also, does freezing your bacteria without glycerol kill it? I know I should know all these things and I'm really sorry, but I'm new to this. Worst case scenario, ...

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... of after ejaculation at most only live in 24 to 72 hours. But if the temperature drop, semen can be stored a few weeks; If use liquid nitrogen freezing, long-term sperm or even to preserve. Sperm survive conditions of temperature is very strict. Only less than body temperature (testicular temperature ...

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