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Dictionary » F » Freezing FreezingFreezing Tending to freeze; for freezing; hence, cold or distant in manner. Freezing machine. See ice machine, under ice. Freezing mixture, a mixture (of salt and snow or of chemical salts) for producing intense cold. Freezing point, that degree of a thermometer at which a fluid begins to freeze; applied particularly to water, whose freezing point is at 32 deg Fahr, and at 0 deg centigrade. ![]()
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Results from our forumthaw cellsJust put them on ice. I saw recently recomendation to keep them on freezing block (-20°C) to keep them alive longer, but I haven't tried it.
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Salmonela E-coli and others - for experts onlyyes bacteria generally survive freezing, but it is better to preserve them in 10-20% glycerol and yes they survive in the stomach
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PCR from cDNA question... that mRNA is stable for your genes of interest. Possible solution -- increase number of cycles. 2. Primers. If you used primers many times before, freezing-thawing can affect their quality. Making new primers from stocks can help. I assume you checked specificity but might be worth looking again. ...
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I made a stupid mistake and need urgent help... colonies? I mean do I need to re-plate them or can I just use a small amount of the liquid that I saved to grow overnight in LB broth? Also, does freezing your bacteria without glycerol kill it? I know I should know all these things and I'm really sorry, but I'm new to this. Worst case scenario, ...
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Re: How long can they live outside the body?... of after ejaculation at most only live in 24 to 72 hours. But if the temperature drop, semen can be stored a few weeks; If use liquid nitrogen freezing, long-term sperm or even to preserve. Sperm survive conditions of temperature is very strict. Only less than body temperature (testicular temperature ...
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