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Freezing

Freezing

Tending to freeze; for freezing; hence, cold or distant in manner. Freezing machine. See ice machine, under ice. Freezing mixture, a mixture (of salt and snow or of chemical salts) for producing intense cold. Freezing point, that degree of a thermometer at which a fluid begins to freeze; applied particularly to water, whose freezing point is at 32 deg Fahr, and at 0 deg centigrade.


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Re:

... are some examples. These emotions are associated with slow breathing and heart-rate, slow visual and verbal processing. These emotions require freezing of thought - freezing of visuals and words - huge amounts of gaps between thinking - to evoke, intensify and sustain.

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by sushil_yadav
Sat Mar 08, 2014 4:20 am
 
Forum: Ecology
Topic: Industrial Society Destroys Mind and Environment
Replies: 45
Views: 32860

Re: Dioxin and Heated Bottled Water

... in plastics, with their formation occuring over 750 degrees F. The myth that water bottles release dioxins in response to ambient heat or freezing conditions has been dispelled by a researcher nearly a decade ago. http://www.jhsph.edu/news/stories/2004/halden-dioxins-two.html

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by Coelacanth
Sun Oct 13, 2013 6:39 am
 
Forum: General Discussion
Topic: Dioxin and Heated Bottled Water
Replies: 1
Views: 726

thaw cells

Just put them on ice. I saw recently recomendation to keep them on freezing block (-20°C) to keep them alive longer, but I haven't tried it.

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by JackBean
Fri Feb 01, 2013 10:16 am
 
Forum: Molecular Biology
Topic: thaw cells
Replies: 1
Views: 576

Salmonela E-coli and others - for experts only

yes bacteria generally survive freezing, but it is better to preserve them in 10-20% glycerol and yes they survive in the stomach

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by triton
Wed Aug 22, 2012 7:12 pm
 
Forum: Microbiology
Topic: Salmonela E-coli and others - for experts only
Replies: 10
Views: 3573

PCR from cDNA question

... that mRNA is stable for your genes of interest. Possible solution -- increase number of cycles. 2. Primers. If you used primers many times before, freezing-thawing can affect their quality. Making new primers from stocks can help. I assume you checked specificity but might be worth looking again. ...

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by dustman
Mon Jun 25, 2012 2:09 pm
 
Forum: Molecular Biology
Topic: PCR from cDNA question
Replies: 1
Views: 2570
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