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Freezes

Freeze substitution

a modification of the freeze-drying method in which the ice within the frozen tissue is replaced by alcohol or other solvent at a very low temperature.


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Will a brain transplant ever be possible

... reason that sometimes we can survive drowning at v. v. low temps. To be honest, I can't remember why the cells don't burst as the water content freezes - maybe they inject some kind of antifreeze??! That said, I think the problem with cryogenic preservation is that you don't exactly know what ...

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by Julie5
Tue Nov 30, 2010 8:41 am
 
Forum: General Discussion
Topic: Will a brain transplant ever be possible
Replies: 7
Views: 4285

I just want to make sure that my hw problems are correct.thk

Why does water expand upon freezing? a. Dissolved air is released from the water, making bubbles in the ice that cause it to expand b. When water freezes, the covalent bonds lengthen and crystals form. c. When water freezes, it forms a crystalline structure in which the hydrogen bonds are perfectly ...

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by maya786
Tue Sep 16, 2008 2:22 am
 
Forum: General Discussion
Topic: I just want to make sure that my hw problems are correct.thk
Replies: 3
Views: 4683

Porblems making DIG probe - please help

... I bet Sp6 wouldn't like dNTPs one bit. ⋅ How's the buffer? Ours (possibly from the mMessage mMachine kit) forms a flocculate whenever it freezes that is difficult to resuspend - it has to be heated and vortexed. The reason I ask is that someone in our lab was spinning it down (!) and ...

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by snowcapk
Tue Jul 29, 2008 7:47 am
 
Forum: Molecular Biology
Topic: Porblems making DIG probe - please help
Replies: 5
Views: 4975

Re: Help me with this Biology home work? Anyone PLEASE!!!

... A. Neurons C. Skin cells B. Liver cells D. Intestinal cells 15. Because water has a relatively low viscosity, it will A. become heavier as it freezes. B. allow substances to diffuse through it. C. have a low heat conductivity. D. change temperature easily. 16. With respect to the four levels ...

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by giselleposse
Tue Apr 15, 2008 9:29 pm
 
Forum: General Discussion
Topic: Help me with this Biology home work? Anyone PLEASE!!!
Replies: 34
Views: 29412

Re: Protein degradation

... Some will store OK at -20C, but usually you store proteins at -80C if you must freeze them. The problem with freezing at -20C is that the sample freezes slowly (minutes to hours) allowing ice crystals to mingle with the protein phase. When ice thaws, it expands and that can damamge protein. Also, ...

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by blcr11
Sat Feb 02, 2008 1:18 pm
 
Forum: Molecular Biology
Topic: Protein degradation
Replies: 2
Views: 1917
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